Changes in phosphomannosyl ligands correlate with cation-dependent mannose-6-phosphate receptors in rat liver during perinatal development

Biochemical and Biophysical Research Communications
Patricia S RomanoMiguel A Sosa

Abstract

The co-existence of two mannose-6-phosphate receptors (CD-MPR and CI-MPR) in most cell types is still a dilemma to be resolved. In this study, some parameters were measured to explore lysosomal apparatus evolution in rat liver during perinatal development, and establish a possible involvement of CD- and/or CI-MPR in lysosome maturation. Activity of four acid hydrolases was measured in the whole organ at different ages and it was found that N-acetyl-beta-D-glucosaminidase (NAG), beta-galactosidase, and beta-glucuronidase change during development, reaching a peak at the 10th day after birth. These results correlated with the expression and binding properties of CD-MPR previously reported. We also used a method that recognizes phosphomannosylated ligands by using purified biotinylated CI-MPR as a probe, and found that the highest concentrations of ligands also appear around the 10th day. Binding assays were also carried out, incubating endogenous NAG from 10-day-old and adult rats with membranes from their respective ages, and the results indicated that cation-dependent mannose-6-phosphate receptor (CD-MPR) has more impact on trafficking of the enzyme at the 10th day after birth. We concluded that lysosome maturation in the rat l...Continue Reading

References

Aug 1, 1991·Current Opinion in Cell Biology·K von Figura
Jan 1, 1989·Annual Review of Cell Biology·S Kornfeld, I Mellman
Jan 1, 1982·Methods in Enzymology·G G SahagianG W Jourdian
Nov 10, 1995·The Journal of Biological Chemistry·R PohlmannK von Figura
Jul 17, 1995·Biochimica Et Biophysica Acta·A Hille-Rehfeld
Aug 1, 1993·Molecular Reproduction and Development·P NissleyM Sklar
Feb 15, 1993·Analytical Biochemistry·K J ValenzanoP Lobel
Sep 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·U MatznerR Pohlmann
Jan 10, 1997·The Journal of Biological Chemistry·D E Sleat, P Lobel
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·J X KangR A Chandraratna
Jul 20, 2002·Biochemical and Biophysical Research Communications·Patricia S RomanoMiguel A Sosa
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Pradipta GhoshStuart Kornfeld

❮ Previous
Next ❯

Citations

Nov 11, 2008·Experimental Cell Research·Maryssa CanuelCarlos R Morales
Jun 19, 2008·Biochemical and Biophysical Research Communications·Maryssa CanuelCarlos R Morales
Jun 7, 2011·Biochemical and Biophysical Research Communications·Guillermo JofréMiguel A Sosa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.