PMID: 11328448May 1, 2001Paper

Changes in preoptic and hypothalamic levels of progesterone receptor mRNA across the oestrous cycle of the ewe

Journal of Neuroendocrinology
C J ScottI J Clarke

Abstract

We measured the levels of progesterone receptor (PR) mRNA in the hypothalamus and preoptic area (POA) of the ewe across the oestrous cycle. Perfusion-fixed hypothalamic tissue was collected from sheep killed during the luteal and follicular phases and during behavioural oestrus. Blood samples taken at the time of tissue collection verified that the oestrous ewes were undergoing a preovulatory luteinizing hormone (LH) surge. Matched sections were taken from the POA, periventricular nucleus (PeVN), ventromedial nucleus (VMN) and arcuate nucleus of each animal. In situ hybridization was performed using a sheep specific, (35)S-labelled riboprobe for PR and semiquantitative image analysis was conducted on emulsion-dipped slides. The number of silver grains per cell was greater in the VMN and arcuate nucleus of oestrous ewes than in luteal and follicular phase ewes; there was no cyclic variation in the level of PR mRNA expression in the cells of the POA and PeVN. The number of labelled cells per mm2 in the VMN was higher in the oestrous ewes than in luteal phase and follicular phase ewes. The number of labelled cells in the PeVN was also higher in the oestrous ewes than in follicular phase ewes, but there was no cyclic variation in t...Continue Reading

References

Aug 1, 1972·The Journal of Endocrinology·I A CummingF C Greenwood
Oct 1, 1983·The Journal of Clinical Endocrinology and Metabolism·J D HoffS S Yen
Jan 1, 1994·The Japanese Journal of Physiology·K Kuba
Jan 1, 1994·Reproduction, Fertility, and Development·G R Shellam
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·D C SkinnerA Caraty
Sep 1, 1995·Trends in Endocrinology and Metabolism : TEM·I J Clarke

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Citations

Apr 24, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·K PerryM Khalid
May 15, 2007·Hormones and Behavior·C Fabre-Nys, H Gelez
Aug 20, 2003·The European Journal of Neuroscience·J C ThiéryB Malpaux
Sep 6, 2005·Domestic Animal Endocrinology·Iain J ClarkeSueli Pompolo
Nov 24, 2006·American Journal of Physiology. Endocrinology and Metabolism·Christopher R McCartneyJohn C Marshall

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