PMID: 6161226Sep 1, 1980Paper

Changes in prolyl endopeptidase during maturation of rat brain and hydrolysis of substance P by the purified enzyme

Journal of Neurochemistry
T KatoS Sakakibara

Abstract

We determined changes in prolyl endopeptidase activity in developing rat brain. A new and highly sensitive fluorogenic substrate, 7-(succinyl-Gly-Pro)-4-methylcoumarinamide, was used for determination of the enzyme activity. The enzyme activity per brain increased until 2 weeks of age, and then decreased during maturation. The enzyme was purified about 7800-fold from the brain of the rat at 2 or 3 weeks of age. The enzyme has a pH optimum of 5.8 to 6.5, and an approximate molecular weight of 70,000. The enzyme activity was completely inhibited by low concentrations of diisopropylfluorophosphate and partially inhibited by high concentrations of phenylmethanesulphonylfluoride, which are potent serine protease inhibitors. Moreover, thiolblocking agents and some heavy metals also have a strong effect on the activity. Bacitracin was found to be a potent inhibitor, with an IC50 value of 2.5 x 10(-6) M at 0.5 mM of the substrate. The enzyme was proved to hydrolyze the NH2-terminal tetrapeptide. Arg1-Pro2-Lys3-Pro4, from substance P to produce the heptapeptide, Gln5-Gln6-Phe7-Phe8-Gly9-Leu10-Met11-CONH2. The Km value of the hydrolysis of substance P was 1.0 mM. This enzyme may be related to the regulation of substance P in the brain, a...Continue Reading

References

Jan 15, 1979·Biochemical and Biophysical Research Communications·K Kangawa, H Matsuo
Aug 1, 1979·Journal of Neurochemistry·M OrlowskiS Wilk
Oct 1, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·F LembeckR Gamse
Mar 1, 1980·Journal of Neurochemistry·T KatoT Nagatsu
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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Citations

Sep 1, 1989·Cellular and Molecular Neurobiology·E A DelBelA R Martins
May 6, 2011·Journal of Molecular Histology·Shin MatsubaraAtsushi P Kimura
Aug 5, 2009·Journal of Molecular Neuroscience : MN·Katsumichi TakedaTakeshi Kato
Apr 27, 1984·Biochimica Et Biophysica Acta·S MizutaniY Tomoda
Aug 1, 1985·Neuroscience Research·M Okada, T Kato
Feb 1, 1986·Neuroscience Research·T KatoA Shimizu
Sep 1, 1982·Peptides·J M StewartW A Krivoy
Sep 11, 1987·Neuroscience Letters·A R MartinsF G De Mello
Oct 1, 1993·Journal of Dermatological Science·M KusuharaY Sasai
Nov 9, 2000·Psychoneuroendocrinology·M MaesS Scharpe
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·B M QuintoD E Casarini
May 23, 1998·The International Journal of Biochemistry & Cell Biology·D F Cunningham, B O'Connor
Aug 21, 2002·Biological & Pharmaceutical Bulletin·Wataru KobayashiHiroshi Noguchi
Aug 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Timo T MyöhänenPekka T Männistö
Jul 31, 2013·PloS One·Andrew J MitchellAlan Saghatelian
Nov 1, 1983·Neurochemical Research·N L BanikM B Sostek
Jun 11, 1999·Journal of Affective Disorders·M MaesS Scharpé
Dec 1, 1985·Neurochemical Research·A M ArzumanyanT N Akopyan
Mar 23, 2010·Journal of Chemical Neuroanatomy·N AgirregoitiaS Jégou
Jan 2, 2007·Neuropeptides·J A García-HorsmanJ I Venäläinen
Jul 29, 2008·The FEBS Journal·Jofre Tenorio-LarangaJ A García-Horsman
Dec 24, 1984·Brain Research·K KoshiyaT Kato
Jan 29, 1986·Biochemical and Biophysical Research Communications·A TogariT Nagatsu

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