Changes in protein expression in two cholangiocarcinoma cell lines undergoing formation of multicellular tumor spheroids in vitro

PloS One
Carlo MischiatiEnzo Agostinelli

Abstract

Epithelial-to-Mesenchymal Transition (EMT) is relevant in malignant growth and frequently correlates with worsening disease progression due to its implications in metastases and resistance to therapeutic interventions. Although EMT is known to occur in several types of solid tumors, the information concerning tumors arising from the epithelia of the bile tract is still limited. In order to approach the problem of EMT in cholangiocarcinoma, we decided to investigate the changes in protein expression occurring in two cell lines under conditions leading to growth as adherent monolayers or to formation of multicellular tumor spheroids (MCTS), which are considered culture models that better mimic the growth characteristics of in-vivo solid tumors. In our system, changes in phenotypes occur with only a decrease in transmembrane E-cadherin and vimentin expression, minor changes in the transglutaminase protein/activity but with significant differences in the proteome profiles, with declining and increasing expression in 6 and in 16 proteins identified by mass spectrometry. The arising protein patterns were analyzed based on canonical pathways and network analysis. These results suggest that significant metabolic rearrangements occur du...Continue Reading

References

Jan 1, 1969·European Journal of Biochemistry·D Dupourque, E Kun
Aug 1, 1973·European Journal of Biochemistry·J CarbonellA Sols
Nov 1, 1969·Biochemistry·E E PenhoetW J Rutter
Jun 12, 2004·Journal of Biomolecular Screening·Leoni A Kunz-SchughartReinhard Ebner
Jun 25, 2005·Seminars in Cancer Biology·Jong Bin Kim
Oct 11, 2005·Lancet·Shahid A KhanSimon D Taylor-Robinson
Jul 5, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thung-S LaiCharles S Greenberg
Aug 8, 2007·Journal of Cellular Physiology·Honor HugoErik W Thompson
Apr 10, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Leda RoncoroniMaria Teresa Bardella
May 17, 2008·Cancer Research·Tamer T OnderRobert A Weinberg
Feb 18, 2009·Clinical & Experimental Metastasis·George J KlarmannWilliam L Farrar
May 15, 2009·Biochimica Et Biophysica Acta·Rommel A Mathias, Richard J Simpson
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jun 3, 2009·The Journal of Clinical Investigation·Michael Zeisberg, Eric G Neilson
Feb 18, 2010·Annals of Surgical Oncology·Tianyu LiShuguang Wang
Jan 25, 2011·Nature Reviews. Cancer·Rob A CairnsTak W Mak
Mar 9, 2011·Nature Medicine·John R Prensner, Arul M Chinnaiyan
Jun 21, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Monique M NijkampJohan Bussink
Dec 2, 2011·Amino Acids·Alfredo BudillonElena Di Gennaro
Jan 10, 2012·Advances in Enzymology and Related Areas of Molecular Biology·Carlo M BergaminiMartin Griffin
Mar 16, 2012·Journal of Proteome Research·K M McMahonC W Sutton
Mar 24, 2012·Biochimica Et Biophysica Acta·Fausto J RodriguezPanos Z Anastasiadis
May 4, 2012·Clinical Biochemistry·Ponlapat YonglitthipagonBanchob Sripa
Sep 5, 2012·Cellular and Molecular Life Sciences : CMLS·Ester Sánchez-TillóAntonio Postigo
Sep 25, 2012·Cancer Research·Dingcheng GaoVivek Mittal
Nov 28, 2012·Oral Oncology·Ashley SmithQuintin Pan
Jan 21, 2014·Biochimica Et Biophysica Acta·Juan Carlos Gallardo-PérezSara Rodríguez-Enríquez
Feb 27, 2014·Current Opinion in Gastroenterology·Alexandra R GrassianDerek Y Chiang

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Citations

Feb 11, 2016·Metallomics : Integrated Biometal Science·Ekaterina Schreiber-BrynzakBernhard K Keppler
Apr 14, 2016·International Journal of Molecular Sciences·Blendi UraGiuseppe Ricci
Mar 10, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Silvestre VicentVincenzo Cardinale
Aug 23, 2020·Cancers·Annamaria MassaMassimo Aglietta
Mar 18, 2016·Oncology Reports·Blendi UraGiuseppe Ricci
Nov 24, 2020·Expert Opinion on Investigational Drugs·Leonardo BaiocchiGianfranco Alpini
Nov 23, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Keisaku SatoGianfranco Alpini

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Methods Mentioned

BETA
protein assay
Chip
phase contrast
electrophoresis
biopsies
ubiquitination

Software Mentioned

Mascot Generic Format ( MGF
Mascot Search engine
ProteomeWeaver
Ingenuity Pathway Analysis ( IPA )
Ingenuity
Ingenuity Pathways Analysis ( IPA )
MassHunter Qualitative Analysis

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