Changes in quantal size distributions upon experimental variations in the probability of release at striatal inhibitory synapses

Journal of Neurophysiology
J C Behrends, G ten Bruggencate

Abstract

Postsynaptic inhibitory gamma-aminobutyric acid-A (GABAA)-receptor-mediated current responses were measured using simultaneous pre- and postsynaptic whole cell recordings in primary cell cultures of rat striatum. Substitution of Sr2+ for extracellular Ca2+ strongly desynchronized the inhibitory postsynaptic currents (IPSCs), resulting in a succession of asynchronous IPSCs (asIPSCs). The rise times and decay time constants of individual evoked asIPSCs were not significantly different from those of miniature IPSCs that are the result of spontaneous vesicular release of GABA. Thus asIPSCs reflect quantal transmission at the individual contacts made by one presynaptic neuron on the recorded postsynaptic cell. Increasing the concentration of Sr2+ from 2 to 10 mM and decreasing that of Mg2+ from 5 to 1 mM produced an increase in the frequency of asIPSCs consistent with an enhancement of the mean probability of release (Pr). At the same time the amplitude distribution of asIPSCs was shifted toward larger values, whereas responses to exogenously applied GABA on average were slightly decreased in amplitude. Application of the GABAB-receptor agonist baclofen (3-10 microM) strongly reduced the frequency of asIPSC, consistent with a decrea...Continue Reading

References

May 1, 1992·The Journal of Physiology·A I Bain, D M Quastel
Jan 1, 1991·Progress in Neurobiology·W Van der Kloot
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J M BekkersC F Stevens
Jan 1, 1969·The Journal of Physiology·F A DodgeR Rahamimoff
Aug 1, 1993·The Journal of Physiology·I Llano, H M Gerschenfeld
Mar 1, 1995·Journal of Neurophysiology·J M Bekkers, C F Stevens
Jun 1, 1993·Trends in Neurosciences·S M ThompsonM Scanziani
Apr 1, 1995·Neuron·C F Stevens, Y Wang
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y Goda, C F Stevens
Oct 29, 1993·Science·C RosenmundG L Westbrook
Mar 1, 1994·Progress in Neurobiology·M VerhageF H Lopes da Silva
Oct 1, 1994·Physiological Reviews·W Van der Kloot, J Molgó
Apr 1, 1994·Journal of Neuroscience Methods·N AnkriH Korn
Oct 1, 1995·Pflügers Archiv : European journal of physiology·K VogtJ Streit
Jun 1, 1996·Current Opinion in Neurobiology·M Frerking, M Wilson
Aug 15, 1996·The Journal of Physiology·M A Abdul-GhaniP S Pennefather

❮ Previous
Next ❯

Citations

Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Uwe Czubayko, Dietmar Plenz
Sep 19, 2003·Journal of Neurophysiology·Nikolai AxmacherRaphael Ritz
Jan 11, 2007·Journal of Neurochemistry·Ellya A BukharaevaLev G Magazanik
Sep 5, 2001·The Journal of Physiology·D EngelA Draguhn
Oct 4, 2008·The Journal of Physiology·Knut KirmseRosemarie Grantyn
Dec 16, 1998·The Journal of Physiology·E Rumpel, J C Behrends
Mar 16, 2000·Reviews in the Neurosciences·D Sulzer, E N Pothos
Feb 9, 2021·Frontiers in Systems Neuroscience·Sean Austin O Lim, D James Surmeier
Feb 9, 2005·Journal of Neuroscience Methods·S KebirM I Glavinović
Nov 18, 2005·Journal of Neuroscience Methods·D GermainM I Glavinovic
Mar 30, 1999·Biophysical Journal·M A Xu-Friedman, W G Regehr
Apr 16, 2009·Biochimica Et Biophysica Acta·M Tajparast, M I Glavinović

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.