Changes in red blood cell membrane structure in G6PD deficiency: an atomic force microscopy study

Clinica Chimica Acta; International Journal of Clinical Chemistry
Jia TangWeiying Jiang

Abstract

Glucose-6-phosphate dehydrogenase deficiency affects over 400 million people worldwide. The hemolytic anemia in G6PD deficiency is usually triggered by oxidative stress, but the mechanism remains uncertain. We have used atomic force microscopy for studying changes in red blood cell membrane and providing new insights on the mechanism. G6PD activity assay and molecular genetic tests were used for molecular diagnosis. AFM was used to investigate alterations in the ultrastructure of G6PD deficient RBC membranes, the influence of different primaquine concentrations, and the protective effects of vitamin C. Nine variants were identified from 33 G6PD deficient individuals. AFM imaging and quantitative analysis showed that G6PD deficient erythrocytes became heterogeneous and roughness measurements of erythrocyte membranes are increased. G6PD enzyme activity and different mutations may relate with roughness parameters. Furthermore, primaquine induces an increased roughness and height of erythrocyte membrane. Meanwhile, primaquine induces damages to erythrocytes which could be prevented by vitamin C treatment in normal RBCs but not in G6PD deficient erythrocytes. Our research may give valuable information about the status of G6PD defici...Continue Reading

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Citations

Feb 20, 2016·Scientific Reports·Vadivel MasilamaniMohamad Saleh AlSalhi
Oct 31, 2018·International Journal of Environmental Research and Public Health·Mohamad S AlSalhiVadivel Masilamani
Nov 20, 2016·Microscopy Research and Technique·Bogdan OprisanMihaela Iuliana Avadanei
Nov 24, 2016·Protein & Cell·Yong ZengHong-Wen Deng

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