PMID: 16512637Mar 4, 2006Paper

Changes in renal cell apoptosis and inflammatory cytokines in early stage of sepsis in rats

Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
Yu ZhangLi Jiang

Abstract

To investigate the relationship between changes in renal cell apoptosis and inflammatory cytokines in sepsis rats. Sepsis was produced in rats by cecal ligation and puncture. Changes in tumor necrosis factor-alpha (TNF-alpha) and monocyte chemotactic protein-1 (MCP-1) levels in renal tissues were determined by enzyme-linked immunoadsorbent assay (ELISA), respectively. Meanwhile, apoptosis rate was also assessed by Annexin V/PI staining and flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) methods. TNF-alpha and MCP-1 levels in renal tissues were significantly increased in rats with sepsis in the early stage. Apoptosis rate of renal cells increased and reached the highest level at 3 hours after sepsis was established and decreased after 6 hours, and the differences were significant (P<0.05 or P<0.01). TNF-alpha and MCP-1 are cytokines which are activated in early stage of sepsis. The increase in renal cell apoptosis in the early stage of sepsis is related with the release of cytokines.

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases
M P Glauser
Advances in Immunology
C E HackL G Thijs
Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
Yu-xiang ZhangPeng-lin Ma
Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
Jian-cheng XiYi Yang
Archives of Surgery
A S HermreckR I MacArthur
© 2021 Meta ULC. All rights reserved