PMID: 15223782Jun 30, 2004Paper

Changes in retinal pigment epithelial gene expression induced by rod outer segment uptake

Investigative Ophthalmology & Visual Science
Itay ChowersDonald J Zack

Abstract

Rod outer segment (ROS) uptake, a crucial function of the retinal pigment epithelium (RPE), probably involve multiple proteins, yet only a small number have so far been identified. The goal of this study was to find additional genes involved in ROS uptake and degradation by identifying ROS-induced gene expression changes. Human RPE-derived ARPE-19 cells were harvested 3 and 12 hours after addition of bovine ROS. Gene expression profiles were compared with control cultures by using a custom human retina cDNA microarray and were validated by quantitative real-time RT-PCR (QPCR). ROS binding and internalization were quantitated with a fluorescence assay. Alterations in the expression levels of multiple genes (especially ones involved in transcriptional regulation, signal transduction, or protein modification) were detected 3 hours after ROS challenge, whereas by 12 hours most had returned to baseline. QPCR results corroborated the microarray results for seven of the eight genes tested. Time-course QPCR experiments on an independent sample set demonstrated characteristic temporal expression changes for each gene. Protein levels of one of these, plasminogen activator inhibitor-1 (PAI-1), were tested and found to parallel the mRNA ch...Continue Reading

Citations

Feb 24, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sylvie JulienUlrich Schraermeyer
Nov 3, 2005·BMC Ophthalmology·Rajesh K SharmaDianna A Johnson
Feb 22, 2008·PLoS Medicine·Marianne HoussierFlorian Sennlaub
Apr 4, 2006·Experimental Eye Research·Ulrich SchraermeyerKarl-Ulrich Bartz-Schmidt
Jul 12, 2005·Experimental Eye Research·Christine A CurcioHoward S Kruth
May 2, 2014·Experimental Eye Research·Francesca MazzoniSilvia C Finnemann
Sep 25, 2016·Progress in Retinal and Eye Research·Xiaowu GuMichael H Elliott
Aug 6, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Debarshi MustafiKrzysztof Palczewski
Aug 15, 2019·Scientific Reports·Nemanja MilićevićMarie-Paule Felder-Schmittbuhl

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