Changes in rhizosphere bacterial gene expression following glyphosate treatment

The Science of the Total Environment
Molli M NewmanJoseph W Kloepper

Abstract

In commercial agriculture, populations and interactions of rhizosphere microflora are potentially affected by the use of specific agrichemicals, possibly by affecting gene expression in these organisms. To investigate this, we examined changes in bacterial gene expression within the rhizosphere of glyphosate-tolerant corn (Zea mays) and soybean (Glycine max) in response to long-term glyphosate (PowerMAX™, Monsanto Company, MO, USA) treatment. A long-term glyphosate application study was carried out using rhizoboxes under greenhouse conditions with soil previously having no history of glyphosate exposure. Rhizosphere soil was collected from the rhizoboxes after four growing periods. Soil microbial community composition was analyzed using microbial phospholipid fatty acid (PLFA) analysis. Total RNA was extracted from rhizosphere soil, and samples were analyzed using RNA-Seq analysis. A total of 20-28 million bacterial sequences were obtained for each sample. Transcript abundance was compared between control and glyphosate-treated samples using edgeR. Overall rhizosphere bacterial metatranscriptomes were dominated by transcripts related to RNA and carbohydrate metabolism. We identified 67 differentially expressed bacterial transcr...Continue Reading

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Nov 19, 2015·The Science of the Total Environment·Molli M NewmanJoseph W Kloepper
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Citations

Sep 3, 2017·Applied and Environmental Microbiology·Daniel C SchlatterTimothy Paulitz
Oct 30, 2016·Applied and Environmental Microbiology·Grace PoldKristen M DeAngelis
Feb 2, 2018·Environmental Sciences Europe·Daisy A MartinezMargaret C Graham
Nov 7, 2019·Molecular Plant-microbe Interactions : MPMI·Bridget S O'BanionSarah L Lebeis
Jan 3, 2018·Environmental Science and Pollution Research International·Martha MertensWolfram Reichenbecher
Aug 21, 2020·MSphere·Dario X Ramirez-VillacisAntonio Leon-Reyes
Mar 16, 2018·Environmental Science and Pollution Research International·Yehao LiuYonghua Yang
Dec 12, 2019·Frontiers in Microbiology·Kyle S BoniferTodd B Reynolds
Apr 3, 2021·Current Microbiology·Keren Hernández GuijarroLeonardo Erijman
May 12, 2019·Aquatic Toxicology·Melissa A MorganCynthia K Larive
Jun 4, 2019·The Science of the Total Environment·Clémence Thiour-MauprivezLise Barthelmebs
Apr 18, 2018·The Science of the Total Environment·Keren Hernández GuijarroLeonardo Erijman
Jul 6, 2021·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jing GuoBo Tao

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