PMID: 6161231Oct 1, 1980Paper

Changes in RNA synthesis and messenger RNA content in the cerebellum of rats with graft versus host disease

Journal of Neurochemistry
W S GriffinM R Morrison

Abstract

Cerebellar RNA accumulation, synthesis, and functional capacity was studied in 14-day-old F1 hybrid rats subjected to neonatally induced graft versus host disease (GVHD). There was a decrease in RNA synthetic rate as measured by the uptake of labeled precursors into RNA. The decrease in total cerebellar RNA synthesis was reflected both in a reduced amount of Nissl substance, visible in cresyl violet-stained 10-micron-thick sections of cerebella, and in the total amount of cytoplasmic RNA isolated from individual cerebella from diseased animals compared with control littermates. Analysis of the RNA translational capacity in wheat germ protein synthesizing systems showed that RNA from experimental animals was also biologically less active. Qualitative differences between protein populations in control and diseased animals were analyzed by two-dimensional gel electrophoresis. There were few alterations in the steady state levels of cerebellar protein. However, two-dimensional gel electrophoresis of the peptides synthesized in vitro by RNA from control and diseased animals showed that there were several changes in the relative abundance of some mRNAs between the two RNA populations. These data show that the cerebellar RNA from rats...Continue Reading

References

May 1, 1979·Brain Research Bulletin·W S GriffinM F Pacheco
Jan 1, 1976·Advances in Immunology·S C Grebe, J W Streilein
Apr 3, 1978·Biochimica Et Biophysica Acta·N Prashad, R N Rosenberg
Jul 1, 1978·Brain Research Bulletin·W S GriffinR Chanda
Jun 3, 1977·Biochimica Et Biophysica Acta·M R MorrisonR N Rosenberg
Sep 28, 1978·Nature·W S GriffinC Carrol
Oct 1, 1978·The Journal of Cell Biology·G T Lee, D L Engelhardt
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Jan 19, 1973·Science·A E Beer, R E Billingham
Apr 1, 1973·Journal of Neurochemistry·A J PatelA L Johnson
May 1, 1969·The Journal of Pediatrics·M Winick
Sep 25, 1969·The New England Journal of Medicine·J L NaimanJ B Arey

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Citations

Nov 1, 1980·Brain Research Bulletin·W S GriffinJ R Head
Jun 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·W S GriffinJ R Head
Feb 1, 1982·Journal of Neurochemistry·D Eliyahu, H Soreq
Jul 1, 1983·Annals of Neurology·M R Morrison, R N Rosenberg
Nov 20, 1981·The Journal of Comparative Neurology·W S GriffinJ R Head
May 15, 1981·Analytical Biochemistry·M R Morrison, W S Griffin

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