PMID: 2108073Feb 1, 1990Paper

Changes in Sertoli cell function in vitro induced by nitrobenzene

Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology
G AllenbyP M Foster

Abstract

Nitrobenzene (NB) has been identified as a testicular toxicant in vivo, but its site of action remains unknown. In the present study, the effect of NB on the Sertoli cell was assessed in vitro using Sertoli cell and Sertoli-germ cell cocultures. The parameters measured were the exfoliation of germ cells; the secretion of lactate, pyruvate, and inhibin; and gross cellular morphology. The effect of metadinitrobenzene (mDNB), a related compound which is a known Sertoli cell toxicant, was assessed for comparison. Gross morphological changes including vacuolation of Sertoli cells were observed following treatment of cultures with 10(-3) M NB. Exposure of cocultures to NB also resulted in dose-dependent exfoliation of predominantly viable germ cells. NB (greater than 5 X 10(-4) M) and mDNB at the single dose level used (10(-4) M) stimulated the secretion of lactate and pyruvate significantly by Sertoli cells, an effect that was more marked in the absence of germ cells. Comparable changes were observed in follicle stimulating hormone (FSH)-stimulated cultures. Inhibin secretion by Sertoli cells was also altered by exposure to NB but in a biphasic manner, with low (10(-8) to 10(-6) M) and high (10(-4) to 10(-3) M) doses enhancing inhib...Continue Reading

Citations

Jan 1, 1993·Reproductive Toxicology·J C Lamb, R E Chapin
Oct 6, 2000·Andrologia·T K MonseesJ Hayatpour
May 1, 1992·Journal of Toxicology and Environmental Health·C GregottiE M Faustman
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Chandrima ShahaDurga Prasad Mishra
Jan 24, 2016·Reproductive Toxicology·Keith M GoldsteinThomas K Baker
Jan 26, 2008·International Journal of Andrology·S YoshidaT Shibamoto
Mar 17, 2007·Inhalation Toxicology·Naoka OnoKen Takeda

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