Changes in serum ovotransferrin levels in chickens with experimentally induced inflammation and diseases

Avian Diseases
H XieN C Rath

Abstract

A competitive enzyme immunoassay was developed to measure the changes in serum levels of ovotransferrin (OTF) during inflammation and infectious diseases in chickens. The assay is based on the competition of serum OTF with a fixed concentration of biotin-labeled OTF to bind to a rabbit anti-chicken transferrin antibody immobilized on microtiter wells. After several washing steps, the antibody-bound biotinylated OTF is probed with streptavidin-horseradish peroxidase conjugate (HRP) followed by a colorimetric detection of the HRP activity. The relative changes in the optical density of color are plotted against the competing concentrations of OTF with logarithmic regression to generate a standard curve that is used to determine the concentrations of OTF in unknown samples. Serum had no effect on the measurement of OTE By this method, the time course changes of serum OTF levels in 4-wk-old male broiler chickens that were subjected to inflammation by croton oil injection were measured. The results showed croton oil-induced inflammation elevated serum OTF levels at 16 hr postinjection. OTF levels reached a peak by 72 hr, remained high through 120 hr, and returned to a basal level of olive oil-injected controls by 240 hr. There were ...Continue Reading

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Citations

Oct 16, 2002·Developmental and Comparative Immunology·Hang XieNarayan C Rath
May 26, 2004·The Veterinary Journal·H MurataM Yoshioka
Apr 16, 2013·Avian Pathology : Journal of the W.V.P.A·Zutao ZhouZili Li
Aug 25, 2015·Viral Immunology·Rajesh ChhabraKannan Ganapathy
Aug 23, 2011·Biochimica Et Biophysica Acta·Francesco GiansantiGiovanni Antonini
Jun 13, 2013·Journal of Agricultural and Food Chemistry·Hisham R IbrahimTakeshi Miyata
Feb 9, 2017·Journal of Proteome Research·James McCordDavid C Muddiman
Mar 10, 2017·Food & Function·Lin LiuGaoxiang Zhu
Aug 5, 2014·Cognitive Science·John Turri
Apr 21, 2018·Biological Trace Element Research·Łukasz JaroszMałgorzata Kwiecień
Aug 24, 2019·Journal of Animal Physiology and Animal Nutrition·Lea MiddendorfChristian Visscher
Oct 9, 2007·International Journal of Biological Macromolecules·Hisham R IbrahimTakayoshi Aoki
Sep 19, 2009·Poultry Science·N C RathP Wakenell

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Methods Mentioned

BETA
electrophoresis
protein assay
enzyme-linked immunosorbent assay

Software Mentioned

Excel

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