PMID: 6988324Jan 1, 1980Paper

Changes in some chromatin and cytoplasmic enzymes of perinatal rat hepatocytes during culture

In Vitro
C Guguen-GuillouzoJ Kruh

Abstract

Hepatocytes prepared from rats at various perinatal stages were cultured in selective medium that does not allow fibroblastic cell growth. Cell population remained homogeneous during the culture. Hepatocytes undergo divisions for a period, which varies according to the stage of development of the rat. Light and electron microscope observations showed the presence of numerous cytoplasmic organelles; moreover, hydrocortisone-induced structures similar to bile canaliculi. Chromatin protein kinase decreased rapidly during culture except in samples prepared from 17-day fetuses in which it remained unchanged for 2 days and decreased to a lesser extent afterwards. Chromatin nonhistone proteins were incubated with (gamma-32P) ATP and the phosphorylation pattern analyzed on polyacrylamide gels. Many radioactive peaks were observed in chromatin proteins from 17-day fetuses; they were much lower in proteins than 19-day fetuses. The phosphorylation pattern was analyzed in hepatocytes after 2 days of culture. Many radioactive peaks were observed with proteins from hepatocytes taken from 17-day fetuses; no radioactivity was observed in proteins from 19-day fetuses. This is in contrast with the absence of radioactive peaks in chromatin protei...Continue Reading

References

Feb 2, 1976·European Journal of Biochemistry·J Kruh, L Tichonicky
Apr 15, 1979·The Biochemical Journal·G C YeohI T Oliver
Apr 2, 1979·European Journal of Biochemistry·C Guguen-GuillouzoJ Kruh
Mar 30, 1978·Biochemical and Biophysical Research Communications·M P LeibovitchJ Kruh
Jul 1, 1976·Experimental Cell Research·G L Murison
Mar 1, 1972·The Journal of Cell Biology·H L Leffert, D Paul
Jan 1, 1973·Biochimie·A Hatzfeld, F Schapira
Oct 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·W F Noyes
Apr 1, 1974·Developmental Biology·F ChytilT C Splesberg
Apr 8, 1974·Biochemical and Biophysical Research Communications·C B Chae, D B Carter
Oct 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·E PenhoetW J Rutter
Jan 28, 1971·Biochimica Et Biophysica Acta·M Kamiyama, T Y Wang
Jan 11, 1968·Biochemical and Biophysical Research Communications·W A Susor, W J Rutter
Feb 1, 1962·Experimental Cell Research·W D HILLIS, F B BANG

Citations

Feb 1, 1993·American Journal of Obstetrics and Gynecology·H AndoY Tomoda
Feb 1, 1981·Journal of Ultrastructure Research·D BourelA Guillouzo
Mar 28, 1980·Biochemical and Biophysical Research Communications·C Guguen-GuillouzoA Kahn
Jan 1, 1980·Annals of the New York Academy of Sciences·C Guguen-GuillouzoJ Kruh
May 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·M J Gómez-LechónJ V Castell
Jul 28, 2006·Integrated Environmental Assessment and Management·Mark A Harwell, John H Gentile
Dec 15, 1980·International Journal of Cancer. Journal International Du Cancer·G E Milo, J A DiPaolo

Related Concepts

Striadyne
Senility
Metazoa
Autoradiography
Cell Division Phases
Chromatin
Chromosomal Proteins, Non-Histone
Protoplasm
Electrophoresis, Agar Gel
Fetal Structures

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