Changes in sperm glycogen synthase kinase-3 serine phosphorylation and activity accompany motility initiation and stimulation

Journal of Andrology
Payaningal R SomanathSrinivasan Vijayaraghavan

Abstract

Sperm motility is regulated by protein phosphorylation. We have shown that the signaling kinase, glycogen synthase kinase-3 alpha (GSK-3 alpha), is present in spermatozoa. In somatic cells, GSK-3 is regulated by serine and tyrosine phosphorylation. In this report, we document that both GSK-3 alpha and GSK-beta isoforms are present in spermatozoa, with GSK-3 alpha being the predominant isoform. The relationship between GSK-3 serine phosphorylation and motility was investigated. Serine phosphorylation of GSK-3 increases significantly in spermatozoa during their passage through the epididymis. Initiation and stimulation of motility in vitro by isobutyl-methyl-xanthine, 2-chloro-2'-deoxy-adenosine, and calyculin A lead to a dramatic increase in GSK-3 serine phosphorylation. The concentration-dependent induction of motility by calyculin A is closely associated with GSK-3 serine phosphorylation. Immunoprecipitation of GSK-3 alpha and GSK-3 beta shows that both of the GSK-3 isoforms are more active in caput than in caudal spermatozoa. Calyculin A treatment decreased the activity of both isoforms. Column chromatography was used to purify inactive GSK-3 alpha from the caudal sperm extracts. This GSK-3 alpha species was phosphorylated at...Continue Reading

References

Oct 24, 1998·Analytical Biochemistry·W J RyvesA J Harwood
Apr 21, 1999·Current Opinion in Cell Biology·S J LeeversM D Waterfield
Dec 2, 1999·Experimental Cell Research·B Vanhaesebroeck, M D Waterfield
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·X FangG B Mills
Nov 10, 2001·Trends in Biochemical Sciences·D P Brazil, B A Hemmings

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Citations

Jun 26, 2007·Asian Journal of Andrology·Rumela ChakrabartiSrinivasan Vijayaraghavan
Jan 25, 2011·Molecular Human Reproduction·Margarida FardilhaEdgar da Cruz E Silva
Oct 29, 2010·Veterinary Medicine International·Julieta CaballeroRobert Sullivan
Nov 20, 2016·Molecular Reproduction and Development·Michael Belenky, Haim Breitbart
Mar 27, 2018·Journal of Cellular Physiology·Souvik DeySrinivasan Vijayaraghavan
Jun 29, 2018·The Journal of Reproduction and Development·Ayano YamadaHiroshi Harayama
Aug 26, 2018·Journal of Cellular Physiology·Suranjana GoswamiSrinivasan Vijayaraghavan
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Souvik DeySrinivasan Vijayaraghavan
Jan 11, 2020·Frontiers in Cell and Developmental Biology·Souvik DeySrinivasan Vijayaraghavan
Jan 24, 2006·Molecular and Cellular Biology·Tsuyoshi KobayashiAkira Kikuchi
Aug 22, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·I M AparicioL J Garcia-Marin
Sep 14, 2007·Archives of Andrology·Yibing HanHuai L Feng
Jan 9, 2015·Biology of Reproduction·Rahul BhattacharjeeSrinivasan Vijayaraghavan
Aug 4, 2020·The Journal of Toxicological Sciences·Lu YuanXiujun Zhang
Oct 28, 2011·Molecular Reproduction and Development·Mariano G BuffoneGeorge L Gerton
Feb 16, 2007·Biology of Reproduction·Rumela ChakrabartiSrinivasan Vijayaraghavan
May 1, 2014·Spermatogenesis·Pranitha Jenardhanan, Premendu P Mathur
Jun 27, 2018·Frontiers in Cell and Developmental Biology·Darya A TourzaniMaría G Gervasi
Feb 1, 2018·Biology of Reproduction·Rahul BhattacharjeeSrinivasan Vijayaraghavan
Nov 18, 2005·Biology of Reproduction·Sonya M SchuhDonner F Babcock
Oct 16, 2015·Systems Biology in Reproductive Medicine·Brett R Lackey, Sandra L Gray
Mar 27, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew T ReidBrett Nixon
Mar 3, 2019·Molecular Human Reproduction·M J FreitasS Vijayaraghavan
Oct 8, 2020·International Journal of Molecular Sciences·Nailis SyifaTzu-Hua Wu

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