Changes in sputum composition during sputum induction in healthy and asthmatic subjects

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
O HolzH Magnussen

Abstract

Induced sputum is increasingly used to characterize the cellular and biochemical composition of the airways. We studied whether the composition of induced sputum is different between samples obtained sequentially during one sputum induction. Subjects with mild asthma (n=7) or healthy subjects (n=6) produced sputum during and after three consecutive 10 min periods of hypertonic saline inhalation. Samples were analysed separately for the three periods. To determine the reproducibility of the cellular composition, sputum induction was repeated on another two days. The mean percentage of neutrophils decreased significantly (P<0.01) during sputum induction in asthmatic (36.9, 29.8, 16.3%) and healthy subjects (43.6, 17.2, 18.0%). Correspondingly, percentages of macrophages increased and percentages of eosinophils were 4.9, 3.5, and 3.7% in the asthmatic and 0.6, 0.7, and 0.5% in the healthy subjects, without significant change over the three periods; mean eosinophil numbers were significantly higher in the subjects with asthma (P< 0.05). Reproducibility of percentage cell counts did not markedly depend on sampling periods in terms of coefficients of variation. The concentration of eosinophil cationic protein decreased in both groups...Continue Reading

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Citations

Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·Peter A B Wark, Peter G Gibson
Sep 26, 2000·Respiratory Medicine· HolzH Magnussen
Sep 3, 2005·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Daniel PohlKai Michael Beeh
Aug 25, 2000·Current Opinion in Pulmonary Medicine·D OlivieriA Chetta
Mar 2, 1999·American Journal of Respiratory and Critical Care Medicine·O HolzH Magnussen
Nov 11, 1999·American Journal of Respiratory and Critical Care Medicine·F E Hargreave, R Leigh
Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·A SpanevelloG Michetti
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·C TaubeH Magnussen
Sep 11, 2001·American Journal of Respiratory and Critical Care Medicine·A ten BrinkeE H Bel
Feb 3, 2006·Environmental Health Perspectives·Jeffrey RattoJohn Balmes
Jan 15, 2013·Respiratory Medicine·Frauke PedersenTorsten Goldmann
Nov 7, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P A WarkP G Gibson
Sep 1, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·O HolzH Magnussen
May 11, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L GrönkeH Magnussen
May 8, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E M ErinT T Hansel
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