PMID: 16647007May 2, 2006Paper

Changes in substance P in myocardium and dorsal root ganglion following coronary artery occlusion in rats

Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
Jian-wen Zhang, Zheng Guo

Abstract

To observe the changes in substance P in myocardium and dorsal root ganglion (DRG) following ligation of the left anterior descending branch of coronary artery. The substance P is commonly considered released mainly from afferent nerve fibers under noxious stimulation and could induce neurogenic inflammation in peripheral tissue. Twenty-four adult male Sprague-Dawley rats, weighing 270-300 g, were randomly divided into four groups: sham-operated group, coronary artery occlusion (CAO) 1 hour group, CAO 3 hours group and CAO 6 hours group, and 6 animals were in each group. The left anterior descending branch of coronary artery was occluded to establish the acute myocardial ischemia model except in the sham-operated group. The myocardium in ischemic and non-ischemic (the contralateral side of ischemic myocardium) region and DRG were harvested as scheduled and processed for immunohistochemistry and for substance P mRNA semi-quantitative examination using reverse transcription-polymerase chain reaction (RT-PCR) technique. (1) substance P and substance P mRNA levels in myocardium of ischemic areas and myocardium of non-ischemic areas were significantly elevated after CAO compared with sham-operated group (all P<0.05), and they were h...Continue Reading

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