Changes in surfactant phospholipids in fetal rat lungs from normal and diabetic pregnancies

Pediatric Research
M RieutortJ R Bourbon

Abstract

The purposes of this study were to adapt and evaluate further a pulmonary surfactant isolation method applicable to unperfused fetal rat lung, to quantitate key phospholipids phosphatidylcholine (disaturated phosphatidylcholine, and phosphatidylglycerol) of the isolated material during the last 3 days of gestation, and to determine if abnormalities in surfactant phospholipids were present in fetuses of diabetic pregnancies. A simplified scheme of sucrose gradient centrifugation proved useful for small scale preparations of material enriched in the phospholipids most characteristic of pulmonary surfactant. It was shown that fetal blood phospholipids did not contaminate the surfactant fraction and therefore would not produce artifacts in assessment of lung maturational changes. Analyses of subcellular fractions isolated at 19.5, 20.5, and 21.5 days revealed that the percentages of disaturated phosphatidylcholine relative to total phospholipids were 23-44% in the surfactant preparations and 14-21% in the residual (nonsurfactant) fractions, while the disaturated phosphatidylcholine/phosphatidylcholine ratios were 0.62 +/- 0.06 and 0.41 +/- 0.03, respectively. Summation of the amounts of individual phospholipids in the two fractions...Continue Reading

Citations

Mar 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·E DoucetC Tordet
Jan 13, 1987·Biochimica Et Biophysica Acta·J R BourbonM Rieutort
Feb 1, 1991·American Journal of Obstetrics and Gynecology·B L TaborJ F Lewis
Jul 1, 1995·Obstetrical & Gynecological Survey·R P Verma
Aug 1, 1992·American Journal of Respiratory Cell and Molecular Biology·S H GuttentagJ Floros
Nov 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C FraslonC Valenza
Mar 1, 1989·Experimental Lung Research·N GuettariC Tordet
Jul 1, 1991·Experimental Lung Research·J R BourbonJ M Johnston
Nov 1, 1993·Experimental Lung Research·I H Gewolb
May 1, 1997·Experimental Lung Research·I H Gewolb, J O'Brien
Sep 26, 2016·Paediatric Respiratory Reviews·M B AzadV W Dolinsky
Jun 11, 2010·Endocrine Reviews·Alicia Jawerbaum, Verónica White

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