Changes in T cell effector functions over an 8-year period with TNF antagonists in patients with chronic inflammatory rheumatic diseases

Scientific Reports
Ilaria SauzulloClaudio Maria Mastroianni

Abstract

The aim of the study was to clarify the effect of long-term anti-TNF therapy on T cell function in patients with rheumatologic immune-mediated inflammatory diseases (IMID). The production of IFNγ by T cells was evaluated at baseline and after 1, 2, 4, and 8 years of anti-TNF agents by means of a QuantiFERON-TB Gold In-Tube assay. The T cell proliferation and surface co-expression of CD25/CD134 in response to phytohaemagglutinin together with the in vitro impact of anti-TNF therapy on the functional capacity of T cells were evaluated after 8 years from the onset of the biological treatment. Age-matched healthy donors were enrolled as controls. The quantitative mitogen-induced IFNγ responses significantly increased with respect to baseline at each time point, apart from the determination after 4 years. We found an increased expression of CD25/CD134 in CD4+ compared to CD8+ T cells both in patients and controls. The in vitro addition of anti-TNF agents induced a significant decrease of both the IFNγ response and of CD25/CD134, whereas no effect on the intensity of the proliferative response was observed. Our data provide a biological basis for the reassuring issues on the safety of long-term anti-TNF treatment in patients with IMID.

References

Mar 13, 2001·Annual Review of Immunology·M Feldmann, R N Maini
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Jul 25, 2006·Arthritis Research & Therapy·Haïfa HamdiUNKNOWN RATIO (Recherche sur Anti-TNF et Infections Opportunistes) Study Group
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Feb 22, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Francesca BosèEva Reali

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Methods Mentioned

BETA
biopsies
flow cytometry
FACS
flow
density-gradient centrifugation
blood draw

Software Mentioned

Flow Jo
MacKiev
FlowJo
GraphPad Prism

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