PMID: 6402517Jan 1, 1983Paper

Changes in the amounts of cytoskeletal proteins within the perikarya and axons of regenerating frog motoneurons

The Journal of Cell Biology
D V Sinicropi, D L McIlwain

Abstract

Changes in the amounts of tubulin, actin, and neurofilament polypeptides were found in regenerating motoneurons of grass frogs during the period of axonal elongation. Ventral roots 9 and 10 were transected unilaterally about 7 mm from the spinal cord. 35 d later, [3H]colchicine binding had decreased in the proximal stumps to approximately one-half of contralateral control values, well before the regenerating motor axons had reinnervated skeletal muscles of the hind limb. [3H]colchicine binding did not change significantly in the operated halves of the 9th and 10th spinal cord segments over a 75-d period. The relative amounts of actin, tubulin, and neurofilament polypeptides in the operated ventral roots were measured by quantitative densitometry of stained two-dimensional electrophoretic gels. Alpha-tubulin, beta-tubulin, and the 68,000 molecular weight subunit of neurofilaments (NF68) decreased within the transected ventral roots to 78%, 57%, and less than 15% of control values, respectively. The amount of actin increased to 132% of control values within the operated ventral roots, although this change was not statistically significant. Opposite changes were found within motoneuronal cell bodies isolated from the spinal cord. ...Continue Reading

References

Jan 1, 1978·The International Journal of Biochemistry·A G Castle, N Crawford
Dec 15, 1978·Brain Research·P B Farel
May 1, 1979·Journal of Neuropathology and Experimental Neurology·L C CorkR J Adams
Nov 1, 1977·Journal of Neurochemistry·D E WeilD L McIlwain
Nov 1, 1978·FEBS Letters·I Gozes, C Richter-Landsberg
Dec 22, 1978·Brain Research·S HesterD L McIlwain
Jul 15, 1976·The Journal of Comparative Neurology·M Murray
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A M Heacock, B W Agranoff
Nov 11, 1975·The Journal of Comparative Neurology·W H VanceR E Coggeshall
Sep 1, 1975·Experimental Neurology·B Grafstein
May 1, 1975·Journal of Neuropathology and Experimental Neurology·K D BarronJ E Mincy
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Apr 1, 1972·The Journal of Cell Biology·D L Price, K R Porter
Jan 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K L SimsV M Pickel
Sep 1, 1968·Neurology·S Carpenter
Jun 1, 1971·The Journal of Cell Biology·K M YamadaN K Wessells
Jun 1, 1971·Journal of Neurochemistry·V N Luine, F C Kauffman
Nov 1, 1973·Journal of Ultrastructure Research·E J Nathaniel, D R Nathaniel
Jun 1, 1966·Journal of Neurochemistry·W E Watson
Apr 1, 1966·Journal of Neurochemistry·W T Norton, L A Autilio
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Jul 1, 1981·Journal of Neurochemistry·J H Skene, M Willard
Nov 1, 1981·Journal of Neurochemistry·G W Perry, D L Wilson
Apr 1, 1982·Journal of Neurochemistry·D V SinicropiD L McIlwain
Nov 1, 1981·Journal of Neurochemistry·L Autilio-GambettiP Gambetti
Aug 1, 1981·Computers and Biomedical Research, an International Journal·B F AycockD L McIlwain
Jan 1, 1967·The Journal of Cell Biology·A Peters, J E Vaughn
May 7, 1957·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·E G GRAY
Jul 1, 1964·Journal of Neuropathology and Experimental Neurology·E A MACKEYJ WIENER

❮ Previous
Next ❯

Citations

Nov 1, 1988·Journal of the Neurological Sciences·R Pamphlett
Jun 1, 1994·Journal of Neuroscience Methods·D L McIlwain, V B Hoke
Jun 1, 1988·Brain Research·M E GoldsteinW W Schlaepfer
Apr 18, 2002·Journal of the Neurological Sciences·Catherine FressinaudFrederic Dubas
Dec 31, 1997·Neurochemistry International·R HaoR F Pfeiffer
Aug 1, 1984·The Journal of Cell Biology·P N HoffmanD L Price

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.