PMID: 9546945Jan 1, 1997Paper

Changes in the amplitude of cyclic load biphasically modulate endothelial cell DNA synthesis and division

Vascular Medicine
G R UpchurchA J Banes

Abstract

Several physical factors, including shear stress and cyclic load, modulate the ability of endothelial cells to respond to injury. The objective of these experiments was to test the hypothesis that cyclic mechanical load stimulates endothelial cell DNA synthesis and division in vitro. Rabbit aortic endothelial cells were cultured on Flex I flexible-bottomed culture plates, and subjected to load amplitudes of increasing magnitude (0, 0.18, 0.24 and 0.27 load at 1 Hz) using a Flexercell strain unit. Cells were harvested enzymatically and cell numbers determined on days 1, 3 and 5 after initiating the load regimen. DNA synthesis was quantified after trichloroacetic acid precipitation of [3H]thymidine-labeled cells from: (1) whole culture wells and (2) areas of minimum and maximum strain in culture cells. Data were analyzed using analysis of variance and a Tukey's test (n = 6 observations/strain regimen per day in triplicate). Results from analysis of endothelial cells in whole, subconfluent cultures showed that cells subjected to strains of 0.18 had a decreased rate of cell division (76% of control) and DNA synthesis (63% of control), while cells subjected to strains of 0.24 and 0.27 had an increased rate of cell division (108 and ...Continue Reading

References

Mar 1, 1976·Experimental Cell Research·C C HaudenschildM Klagsbrun
Jan 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A Gupte, J A Frangos
Jun 1, 1989·Journal of Cellular Physiology·S F GorfienE J Macarak
Dec 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M J PeadL E Lanyon
Jun 1, 1988·The Journal of Surgical Research·B E Sumpio, A J Banes
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P F DaviesM A Gimbrone
Nov 1, 1973·The Journal of Clinical Investigation·E A JaffeR L Nachman
Dec 1, 1969·The American Journal of Physiology·P B Dobrin, A A Rovick
Aug 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·A van GrondelleN F Voelkel
Oct 1, 1983·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·M L EllsworthJ C Newell
Jul 1, 1984·Microvascular Research·S G EskinL T Navarro
Aug 1, 1981·Journal of Biomechanical Engineering·C F DeweyP F Davies
Nov 1, 1964·Archives of Surgery·J A DEWEESEC G ROB

❮ Previous
Next ❯

Citations

Sep 19, 2003·Tissue Engineering·Sean J KirkpatrickDonald D Duncan
Feb 13, 2003·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·J E WoodellR H Hilderman
Mar 18, 2005·American Journal of Physiology. Cell Physiology·Cornelia HaselPeter Möller
Feb 4, 2009·Journal of Cardiovascular Pharmacology·Victoria E A Stoneman, Martin R Bennett
Aug 18, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michelle WallAlbert J Banes
Oct 7, 2021·Biomechanics and Modeling in Mechanobiology·Cansu KarakayaSandra Loerakker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.