Jan 1, 1995

Changes in the arrangement of actin filaments in myoid cells and Sertoli cells of rat testes during postnatal development and after experimental cryptorchidism

The Anatomical Record
M MaekawaT Nagano

Abstract

Abundant actin filaments are present in myoid cells and Sertoli cells in the testis. In the adult rat, the filaments form a lattice arrangement within the myoid cell, and show a hexagonal pattern in the basal junctional regions of Sertoli cells. Isolated seminiferous tubules and frozen sections were prepared from juvenile to adult Wistar rat testes, stained with FITC-conjugated phalloidin, and observed by confocal microscopy. Unilateral cryptorchidism was induced in adult rats, and seven days later, their testes were also examined. In the myoid cell, parallel actin filaments running circularly around the seminiferous tubules were observed at 15 and 20 days of age. Then, at 30 days, actin filaments arranged longitudinally along the tubular long axis appeared in addition to the circular bundles. A lattice arrangement of actin-filament bundles in myoid cells became obvious at 40 days, when elongated spermatids are found in the tubule. Actin filaments in the basal junctional regions of Sertoli cells did not acquire the hexagonal pattern seen in the adult testis until 30 days of age. In the cryptorchid testes, the arrangement of actin filaments in the both cells showed a remarkable change compared to the control testis; the filament...Continue Reading

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Citations

Mentioned in this Paper

Benign Neoplasm of Testis
Cryptorchidism
Isoactin
Neoplasm of Uncertain or Unknown Behavior of Testis
Actins
Structure of Sertoli Cell
Inferior Colliculus
Testis
Seminiferous Tubule Structure
Electron Microscopy