Changes in the carboxyl-terminal repeat region affect extracellular activity and glucan products of Streptococcus gordonii glucosyltransferase.

Infection and Immunity
M M VickermanD B Clewell

Abstract

Glucans produced by the glucosyltransferase (GTF) of Streptococcus gordonii confer a hard, cohesive phenotype (Spp+) on colonies grown on sucrose agar plates. S. gordonii strains with specific mutations in the region of gtfG that encodes the GTF carboxyl terminus were characterized. In the parental strain Challis CH1, this region included a series of six direct repeats thought to function in glucan binding. The spontaneous mutant strain CH107 had a 585-bp deletion resulting in the loss of three internal direct repeats. Insertional mutagenesis was used to construct strain CH2RPE, which had the parental repeat region but was missing 14 carboxyl-terminal amino acids. The similarly constructed strain CH4RPE had an in-frame addition of 390 nucleotides encoding two additional direct repeats. Although strains CH1, CH2RPE, and CH4RPE all had similar levels of extracellular GTF activity, strain CH107 had less than 15% of the parental activity; however, Western blots (immunoblots) indicated that the amounts of extracellular GTF protein in all four strains were similar. 13C NMR analyses indicated that partially purified GTFs from the Spp+ strains CH1, CH2RPE, and CH4RPE all produced glucans with similar ratios of alpha1,6 and alpha1,3 glu...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1975·Infection and Immunity·B TerleckyjG D Shockman
Sep 1, 1992·Journal of Bacteriology·Y J Nakano, H K Kuramitsu
May 1, 1992·Molecular & General Genetics : MGG·C von Eichel-StreiberM Sauerborn
Dec 1, 1991·Applied and Environmental Microbiology·M M VickermanG W Jones
Mar 1, 1991·Journal of General Microbiology·R J Haisman, H F Jenkinson
Jun 1, 1991·Oral Microbiology and Immunology·E V FrandsenM Kilian
Oct 1, 1990·Journal of General Microbiology·O HondaH K Kuramitsu
Apr 1, 1990·Oral Microbiology and Immunology·R A Buchan, H F Jenkinson
Jan 1, 1990·Archives of Oral Biology·R R Russell
Oct 15, 1985·Carbohydrate Research·D A Grahame, R M Mayer
Sep 1, 1987·Journal of Bacteriology·J J FerrettiR R Russell
Aug 26, 1986·Nucleic Acids Research·M Gribskov, R R Burgess
Apr 27, 1984·Biochimica Et Biophysica Acta·D A Grahame, R M Mayer

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Citations

Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Sacha A F T van HijumIneke G H van Geel-Schutten
Dec 15, 2006·FEMS Microbiology Letters·Jeffrey A BanasM Margaret Vickerman
Mar 11, 2015·Journal of Dental Research·F ZhuH Wu
May 11, 1999·FEMS Microbiology Reviews·V MonchoisP Monsan
Feb 28, 2002·Oral Microbiology and Immunology·M M VickermanC A Edwards
May 24, 2005·Infection and Immunity·Bing Wang, Howard K Kuramitsu
Oct 14, 2006·FEMS Microbiology Letters·Barbara GiomarelliGianni Pozzi
Feb 4, 2014·Journal of Oral Microbiology·Austin RickerAnna Dongari-Bagtzoglou
Mar 21, 1998·Bioscience, Biotechnology, and Biochemistry·K FunaneM Kobayashi
Sep 25, 2004·Bioscience, Biotechnology, and Biochemistry·Kazumi FunaneMikihiko Kobayashi
Mar 12, 2013·TheScientificWorldJournal·Satoko InagakiMichiyo Matsumoto-Nakano
Feb 12, 2020·The ISME Journal·João Gabriel Silva SouzaAnna Dongari-Bagtzoglou
May 24, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J A Banas, M M Vickerman
Aug 1, 2002·Journal of Clinical Microbiology·Wanda B SongyStanley Falkow
Oct 17, 2008·Carbohydrate Research·John F RobytRupendra Mukerjea

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