Changes in the cerebrospinal fluid circulatory system of the developing rat: quantitative volumetric analysis and effect on blood-CSF permeability interpretation

Fluids and Barriers of the CNS
Jean-François Ghersi-EgeaN Strazielle

Abstract

The cerebrospinal fluid (CSF) circulatory system is involved in neuroimmune regulation, cerebral detoxification, and delivery of various endogenous and exogenous substances. In conjunction with the choroid plexuses, which form the main barrier site between blood and CSF, this fluid participates in controlling the environment of the developing brain. The lack of comprehensive data on developmental changes in CSF volume and distribution impairs our understanding of CSF contribution to brain development, and limits the interpretation of blood-CSF permeability data. To address these issues, we describe the evolution of the CSF circulatory system during the perinatal period and have quantified the volume of the different ventricular, cisternal and subarachnoid CSF compartments at three ages in developing rats. Immunohistofluorescence was used to visualize tight junctions in parenchymal and meningeal vessels, and in choroid plexus epithelium of 19-day fetal rats. A quantitative method based on serial sectioning of frozen head and surface measurements at the cutting plane was used to determine the volume of twenty different CSF compartments in rat brain on embryonic day 19 (E19), and postnatal days 2 (P2) and 9 (P9). Blood-CSF permeab...Continue Reading

References

Oct 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A NehligS Boyet
Feb 2, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anne Pereira de VasconcelosAstrid Nehlig
Jun 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Pia KivisäkkRichard M Ransohoff
Sep 2, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Steven R Ennis, Richard F Keep
Dec 14, 2005·Current Topics in Developmental Biology·Zoran B RedzicJoanna Szmydynger-Chodobska
Jun 28, 2006·The European Journal of Neuroscience·P A JohanssonN R Saunders
Feb 23, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Pia A JohanssonNorman R Saunders
Aug 14, 2012·Histochemistry and Cell Biology·Ingrid KratzerJean-François Ghersi-Egea
Aug 7, 2013·Fluids and Barriers of the CNS·Ingrid KratzerJean-Francois Ghersi-Egea
Mar 7, 2014·Translational Psychiatry·E S OnaiviZ C Lin
Jan 1, 1985·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G NormandG Gombos

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Citations

May 24, 2018·American Journal of Physiology. Cell Physiology·Daniel PrestonBonnie L Blazer-Yost
Jan 26, 2018·Acta Neuropathologica·Jean-François Ghersi-EgeaBritta Engelhardt
Mar 9, 2018·Pharmaceutical Research·Jean-François Ghersi-EgeaNathalie Strazielle
Jul 9, 2020·International Journal of Molecular Sciences·Karol Kaiser, Vitezslav Bryja
Jun 28, 2018·American Journal of Physiology. Cell Physiology·Elodie SaudraisJean-François Ghersi-Egea

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Methods Mentioned

BETA
electron

Software Mentioned

ZEN
AxioVs40

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