Changes in the concentration of microvilli on the free surface of healing mesothelium are associated with alterations in surface membrane charge

The Journal of Pathology
S E MutsaersJ M Papadimitriou

Abstract

The luminal plasmalemma of regenerating mesothelial cells was examined by transmission electron microscopy and the concentration of microvilli at various stages of healing was quantified. Charged tracer and lectin binding techniques were also employed to investigate electrostatic and chemical changes in mesothelial glycocalyx. In uninjured mesothelium and at all stages of healing, the concentration of microvilli at the cellular periphery was greater than over the main cell mass (P < 0.05). Furthermore, there was an increase in the concentration of microvilli in all regions by day 4, which reached a maximum at day 6, then at days 10-15 returned to values closer to uninjured mesothelium (P < 0.01). These changes were associated with an alteration in surface charge. In all lesions, the surface charge on microvillar membranes was greater than for flat membranes, but the difference was only significant at days 4, 6, and 15 (P < 0.001). The changes in surface charge may reflect a differential expression of mucopolysaccharides on the surface membrane. In addition, concanavalin A bound avidly to mesothelial surface membranes, suggesting the presence of alpha-methyl-D-mannoside residues. These findings suggest an association between mic...Continue Reading

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Citations

Dec 29, 2011·Journal of Biomedicine & Biotechnology·Susan Yung, Tak Mao Chan
Feb 13, 2002·The American Journal of Pathology·Steven E MutsaersJohn M Papadimitriou
Jan 4, 2017·Matrix Biology : Journal of the International Society for Matrix Biology·Ville KoistinenKirsi Rilla
Aug 3, 2002·Respirology : Official Journal of the Asian Pacific Society of Respirology·Steven E Mutsaers
Apr 30, 2014·Infection and Immunity·Kaila M BennettDavid D Lo
Dec 30, 1999·In Vitro Cellular & Developmental Biology. Animal·J OhanL Drouet
Oct 27, 2007·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·J B C van der Wal, J Jeekel
Jan 29, 2016·Histochemistry and Cell Biology·Ville KoistinenKirsi Rilla
Dec 25, 2019·Pharmaceutical Research·Abdullah Al ShoyaibVardan T Karamyan
Jan 21, 2000·The Journal of Pathology·S E MutsaersJ M Papadimitriou
Sep 18, 2020·PloS One·Willi L WagnerSteven J Mentzer
Sep 30, 2016·World Journal of Gastroenterology : WJG·Lieselotte LemoineKurt Van der Speeten
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Divya J Mekala, Terrence L Geiger
Jul 14, 2007·The International Journal of Artificial Organs·P A Lucas
Jul 14, 2007·The International Journal of Artificial Organs·N Di PaoloG Garosi
Jul 3, 2018·Frontiers in Physiology·Andres Isaza-RestrepoRafael Riveros-Dueñas
Jul 14, 2007·The International Journal of Artificial Organs·S Yung, T M Chan
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Sarah E Herrick, Steven E Mutsaers

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