Changes in the expression of surface receptors on lymphocyte subsets in the elderly: quantitative flow cytometric analysis

American Journal of Hematology
Lia GinaldiD Quaglino

Abstract

The immunophenotype of circulating lymphocytes, including the intensity expression of surface receptors, changes with ageing. Until now, no results of systematic studies on age-dependent changes with respect to the expression of the major lymphocyte surface receptors in healthy elderly subjects have been reported. In order to identify age-related changes in both representation and immunophenotype of lymphocyte populations, we investigated, by means of triple-color whole-blood immunostaining and quantitative flow cytometry, the percent values and the absolute numbers, as well as the levels of surface antigen expression or antigen molecules per cell (ABC values x 10(3)), of different peripheral blood lymphocyte subsets from 23 healthy elderly subjects and 13 young donors. Naive (CD45RA+CD3+) T cells, total B cells, and CD5+ B lymphocytes are decreased (22%, 6%, 0.8% vs. 30%, 12%, 1.4%, respectively), whereas activated (HLA-DR+CD3+) and memory (CD45RO+CD3+) T cells, CD3+CD7- T lymphocytes, and lymphocytes expressing the NK marker CD56 are expanded in the elderly (2%, 53%, 13%, 6% vs. 0.8%, 45%, 8%, 8%, respectively). Moreover, T lymphocytes from elderly individuals express lower CD3 (61 +/- 10) compared to young (69 +/- 10). Consi...Continue Reading

References

Oct 1, 1989·Immunological Reviews·B E Bierer, S J Burakoff
Oct 1, 1994·Clinical and Experimental Immunology·S KukelH W Kreysel
Jan 1, 1995·Immunology Today·C FranceschiA Cossarizza
Jun 1, 1994·Aging : Clinical and Experimental Research·R PaganelliI J Ansotegui
Jan 1, 1996·International Immunology·M RoedererL A Herzenberg
Feb 1, 1996·Seminars in Immunology·X Chen, J F Kearney
Feb 1, 1997·Mechanisms of Ageing and Development·G PawelecI Beckman
Apr 1, 1997·Cytometry·A CossarizzaC Franceschi
Mar 1, 1997·Mechanisms of Ageing and Development·G M Shearer
Jul 1, 1997·Clinical Immunology and Immunopathology·T W McCloskeyS Pahwa
Jun 1, 1997·Mechanisms of Ageing and Development·R Krishnaraj
Jun 15, 1997·Cellular Immunology·M L Thoman
Nov 5, 1997·Journal of Clinical Immunology·C MiyajiT Abo
Nov 14, 1997·American Journal of Clinical Pathology·L GinaldiD Quaglino
Dec 5, 1997·Immunology Today·G Pawelec, R Solana
Feb 26, 1998·Immunological Reviews·P J LintonS Swain
Feb 26, 1998·Immunological Reviews·N R Klinman, G H Kline
May 13, 1998·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·L GinaldiD Quaglino
Aug 26, 1998·Journal of Clinical Pathology·L GinaldiD Catovsky

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Citations

Dec 21, 2004·Biogerontology·Mónica De la FuenteJesus A F-Tresguerres
Dec 18, 2001·Mechanisms of Ageing and Development·Katharina SchindowskiAnne Eckert
Oct 3, 2001·Microbes and Infection·L GinaldiM De Martinis
Jul 21, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Adriana I ColovaiAttilio Orazi
Dec 24, 2005·The Journal of Trauma·Timothy P PlackettElizabeth J Kovacs
Apr 28, 2010·Immunity & Ageing : I & a·Christine V CrooksClare R Wall
Feb 9, 2012·The Indian Journal of Medical Research·Madhu Vajpayee, Teena Mohan
Nov 30, 2010·La Revue de médecine interne·E CrételM Heim
Dec 19, 2009·Cytometry. Part B, Clinical Cytometry·Indu ArunMaryalice Stetler-Stevenson
Feb 8, 2006·Experimental and Molecular Pathology·Massimo De MartinisLia Ginaldi
Jun 19, 2004·Hematology·Leanne Berkahn, Armand Keating
Aug 18, 2005·Veterinary Immunology and Immunopathology·Daniel G BlountPaul R Heaton
Feb 24, 2015·Cytometry. Part B, Clinical Cytometry·Susanne MelzerAttila Tárnok
Apr 15, 2016·The Analyst·Xichen ZhangMarkus Beck
Jun 20, 2002·The Journal of Biological Chemistry·Kate J ClaycombeSimin Nikbin Meydani
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Timothy P PlackettElizabeth J Kovacs
Nov 2, 2002·Scandinavian Journal of Immunology·M Shodell, F P Siegal
Sep 3, 2016·Journal of Hematology & Oncology·Haouraa MostafaE Marion Schneider
Jul 14, 2020·Frontiers in Immunology·Marisa MarketRebecca C Auer
Jan 25, 2020·International Journal of Medical Microbiology : IJMM·M Samiul AlamKristina Williams
Jun 4, 2018·Developmental and Comparative Immunology·Sita S WithersRobert B Rebhun

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