Changes in the Intestinal Microbiota Are Seen Following Treatment with Infliximab in Children with Crohn's Disease

Journal of Clinical Medicine
Kinga Kowalska-DuplagaKrzysztof Fyderek

Abstract

The aim of the study was to determine the impact of biological treatment with tumor necrosis factor α antibodies (anti-TNF-α) on the intestinal microbiome of children with severe Crohn's disease (CD) and to evaluate the differences in the intestinal microbiome between patients treated with biological therapy and healthy children. Microbiota composition was analyzed by 16S next-generation sequencing (NGS) and microbial profiles were compared between studied groups. Fifty-four samples (from 18 patients before and after anti-TNF-α induction therapy and 18 healthy children) were used in the sequencing analysis. Shannon's diversity index (p = 0.003, adj. p = 0.010) and observed operational taxonomic units (OTUs) (p = 0.007, adj. p = 0.015) were different between controls and patients with prior therapy for CD. Statistically significant dissimilarities between beta diversity metrics, indicating distinct community composition across groups, were observed in patients with CD before and after therapy. We did not observe any differences between controls and patients with CD after therapy. Core microbiome analysis at species level showed that 32 species were present only in patients with CD but not in controls. The results show that biolo...Continue Reading

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Citations

Sep 16, 2020·Alimentary Pharmacology & Therapeutics·Henry TaylorAilsa L Hart
Jul 9, 2020·Transplant International : Official Journal of the European Society for Organ Transplantation·Saranya SivarajMamatha Bhat
Mar 7, 2021·Pathogens·Martina FranzinGabriele Stocco
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jakub RuszkowskiJacek M Witkowski

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Methods Mentioned

BETA
biopsies

Software Mentioned

vsearch
QIIME2
ALDEx2
SATé
LEfSe
Greengenes
DADA2
PICRUSt
PICRUSt ( Phylogenetic Investigation of Communities by Reconst...
LEfSe ( Linear Discriminant Analysis Effect Size )

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