Changes in the microRNA expression profile during blood storage

BMJ Open Sport & Exercise Medicine
Anna HaberbergerMichael W Pfaffl

Abstract

For several decades, autologous blood doping (ABD) in sports has been a major problem, and even today there is still no reliable method for satisfactorily detecting ABD. For this kind of doping, stored individual erythrocytes are used to increase stamina and endurance caused by a higher erythrocyte level in the athlete's body. Since there is growing evidence that these cells are enriched with microRNAs (miRNAs), this study has been carried out to discover and validate all miRNAs occurring in fresh blood as well as in stored blood. Therefore, small RNA Next Generation Sequencing has been performed, which allows untargeted detection of all miRNAs in a blood sample. The focus of this investigation has been to find miRNA alterations in blood bags after erythrocyte processing and during storage, as compared with fresh blood directly withdrawn from subjects. Blood samples were obtained from 12 healthy, recreationally active male subjects three times before blood donation and from blood bags at several time points after blood processing. 189 miRNAs have been considered stable over two consecutive weeks. A further analysis revealed a complex biomarker signature of 28 miRNAs, consisting of 6 miRNAs that altered during 6 weeks of storage...Continue Reading

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Citations

Nov 30, 2018·Drug Testing and Analysis·Mario ThevisHans Geyer
Dec 31, 2020·Diagnostics·Anna RocchiIsabella Spinetti
Dec 17, 2020·American Journal of Hematology·Veronika MussackMichael W Pfaffl

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Methods Mentioned

BETA
Assay
PCR
Chip
DNA Assay
Illumina sequencing
PCA
PCAs

Software Mentioned

GenEx
DeSeq2 R script
SAMtools
Bioanalyzer 2100
Bowtie short read aligner
Compomaster
Fast QC

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