PMID: 8611460Apr 1, 1996Paper

Changes in the pattern of distribution of von Willebrand factor in rat aortic endothelial cells following thrombin generation in vivo

British Journal of Haematology
Y SenisA R Giles

Abstract

The pattern of distribution of von Willebrand factor (VWF) in relatively large sheets of rat aortic endothelial cells (EC) obtained by the Häutchen technique were analysed by immunocytochemistry and light microscopy. EC were examined pre and post administration of a procoagulant mixture of factor Xa (F.Xa) and phosphotidylcholine/phosphotidylserine (PCPS) vesicles which was demonstrated to result in the selective loss of high molecular weight multimers (HMWM) of plasma VWF in the rat. In placebo animals the pattern was heterogenous both in overall distribution and in individual cells which showed both a diffuse and granular pattern. Groups of intensely stained EC were oriented parallel to the longitudinal axis of the aorta and staining was particularly prominent around the orifices of the intercostal arteries, implicating shear-stress as a possible factor in VWF expression by EC. Changes in the pattern of distribution of staining were observed at various time points post-infusion of F.Xa/PCPS, suggesting the immediate release of VWF from EC stores followed by the recruitment of EC to synthesize and store VWF. These changes are consistent with the decrease in EC Weibel-Palade Body (WPB) content observed by EM in previously repor...Continue Reading

Citations

Sep 10, 2002·Journal of Hepatology·Yaacov BaruchGiuliano Ramadori
Oct 20, 1998·The American Journal of Pathology·G ThurstonD M McDonald
Oct 15, 2009·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Mustafa AyNermin Gozukirmizi
Jun 6, 2018·Blood·Anna M RandiGiancarlo Castaman
Jan 6, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Andrew R BondPeter D Weinberg
May 23, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·P W KamphuisenR M Bertina
Sep 25, 2003·Physiological Reviews·Bjarne Osterud, Eirik Bjorklid
Apr 1, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T Y HuangC J Jen
Jan 16, 2020·International Journal of Hematology·Yoshiyuki OgawaHiroshi Handa
Feb 13, 2001·Journal of Applied Physiology·M H LaughlinE M Price
Jan 3, 2021·International Journal of Hematology·Toshiaki IbaJerrold H Levy

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