PMID: 2120055Sep 11, 1990Paper

Changes in the phosphorylation state of the inhibitory guanine-nucleotide-binding protein Gi-2 in hepatocytes from lean (Fa/Fa) and obese (fa/fa) Zucker rats

European Journal of Biochemistry
M BushfieldM D Houslay

Abstract

Treatment of intact, 32Pi-labelled hepatocytes from lean Zucker rats with a range of agents including 12-O-tetradecanoyl-phorbol 13-acetate (TPA), vasopressin, and angiotensin II elicited substantial increases in the phosphorylation of the alpha-subunit of the inhibitory G protein of adenylate cyclase (alpha Gi-2). These agonist-induced phosphorylations of alpha Gi-2 were associated with loss of Gi function as assessed by the ability of low concentrations of guanylyl 5'-[beta,gamma imido]triphosphate (p[NH]ppG) to inhibit forskolin-stimulated adenylate cyclase activity. Hepatocytes from obese Zucker rats displayed a resistance to both agonist-induced phosphorylation of alpha Gi-2 and to p[NH]ppG-mediated inhibition of adenylate cyclase. The basal level of alpha Gi-2 phosphorylation in hepatocytes from obese Zucker rats was considerably greater at 1.06 +/- 0.09 mol phosphate/mol alpha Gi-2 than in hepatocytes from lean animals which gave 0.54 +/- 0.09 mol phosphate/mol alpha Gi-2. Incubation with TPA (10 ng/ml, 15 min) approximately doubled the level of phosphorylation of alpha Gi-2 in the hepatocytes from lean animals but had little effect on the phosphorylation of alpha Gi-2 in hepatocytes from obese animals. Incubation of hep...Continue Reading

References

Dec 15, 1978·The Biochemical Journal·S A SmithC I Pogson
Jun 1, 1990·The Biochemical Journal·M BushfieldM D Houslay
Sep 18, 1987·Science·O M Rosen
Jan 1, 1989·Annals of the New York Academy of Sciences·A M BrownL Birnbaumer
Oct 1, 1988·Molecular and Cellular Endocrinology·N Bégin-Heick, J Welsh
Aug 15, 1984·The Biochemical Journal·C M HeyworthM D Houslay
Nov 1, 1989·Trends in Endocrinology and Metabolism : TEM·A M Spiegel

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Citations

Mar 1, 1997·Journal of Cellular Biochemistry·T EbinaY Ishikawa
Sep 29, 1995·The Journal of Biological Chemistry·T A Fields, P J Casey
Mar 22, 1996·The Journal of Biological Chemistry·H PoppletonT B Patel
Oct 3, 1999·Physiological Reviews·A J Morris, C C Malbon

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