Changes in the Plasma Proteome of Manduca sexta Larvae in Relation to the Transcriptome Variations after an Immune Challenge: Evidence for High Molecular Weight Immune Complex Formation.

Molecular & Cellular Proteomics : MCP
Yan HeHaobo Jiang

Abstract

Manduca sextais a lepidopteran model widely used to study insect physiological processes, including innate immunity. In this study, we explored the proteomes of cell-free hemolymph from larvae injected with a sterile buffer (C for control) or a mixture of bacteria (I for induced). Of the 654 proteins identified, 70 showed 1.67 to >200-fold abundance increases after the immune challenge; 51 decreased to 0-60% of the control levels. While there was no strong parallel between plasma protein levels and their transcript levels in hemocytes or fat body, the mRNA level changes (i.e.I/C ratios of normalized read numbers) in the tissues concurred with their protein level changes (i.e.I/C ratios of normalized spectral counts) with correlation coefficients of 0.44 and 0.57, respectively. Better correlations support that fat body contributes a more significant portion of the plasma proteins involved in various aspects of innate immunity. Consistently, ratios of mRNA and protein levels were better correlated for immunity-related proteins than unrelated ones. There is a set of proteins whose apparent molecular masses differ considerably from the calculatedMr's, suggestive of posttranslational modifications. In addition, some lowMrproteins we...Continue Reading

References

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Apr 11, 2015·Insect Biochemistry and Molecular Biology·Xiaolong CaoHaobo Jiang

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Citations

Jun 28, 2016·Insect Biochemistry and Molecular Biology·Fan YangHaobo Jiang
Dec 8, 2020·Frontiers in Plant Science·Kerui HuangXiaocheng Jiang
Apr 23, 2017·Developmental and Comparative Immunology·Xuesong HeHaobo Jiang
Oct 24, 2020·Insect Biochemistry and Molecular Biology·Xiaolong CaoHaobo Jiang

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