PMID: 3214461Jul 1, 1988Paper

Changes in the platelet surface charge in rabbits with experimental hypercholesterolemia

Atherosclerosis
C Lupu, M Calb

Abstract

The effect of hypercholesterolemia on the platelet surface charge was examined in rabbits fed a lipid-rich diet (0.5% cholesterol and 5% butter). The strong anionic sites were detected with cationized ferritin (CF) pI 8.4, and the sialic acid concentration was evaluated by biochemical assays. In normal rabbits (average plasma cholesterol 0.36 +/- 0.05 mg/ml, and total platelet sialic acid 30.03 +/- 6 micrograms/mg protein) the platelet surface displayed a homogeneous distribution of CF, which also labeled the open canalicular system. Beginning with the third week of diet, at a plasma cholesterol level of 4.6 +/- 0.3 mg/ml, a reduction in the overall platelet negative charge was observed. As the diet progressed and the plasma cholesterol level increased, the CF binding to platelet surface diminished up to an almost total disappearance when the plasma cholesterol reached 18 mg/ml (the 20th week of diet). At the same time a progressive decrease in the sialic acid content up to 5.1 micrograms/mg protein was detected. These results suggest that diet-induced hyperlipidemia causes significant alterations in the platelet surface negative charge, especially in the sialic acid content.

References

Nov 15, 1985·Thrombosis Research·M Steiner, S Vancura
Apr 11, 1969·Analytical Biochemistry·A Zlatkis, B Zak
Jun 1, 1964·The American Journal of Physiology·T H SPAET, M B ZUCKER

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Citations

Jan 1, 1991·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·D H ChuiD S Liu
Aug 17, 2002·Thrombosis Research·Robert MandicWolfgang Wesemann
Oct 22, 2010·Journal of Thrombosis and Haemostasis : JTH·N AlexandruA Georgescu
Jul 20, 2007·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Ilknur I BütünAhmet Dirican
Apr 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·C C WuY T Lee
Apr 30, 2017·Experimental and Therapeutic Medicine·Hidemi Hattori, Masayuki Ishihara

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