Changes in the quantity of histone and actin messenger RNA during the development of preimplantation mouse embryos

Developmental Biology
D H GiebelhausG A Schultz

Abstract

Actin and histone H3 mRNA levels in mouse eggs and early embryos have been measured by use of recombinant DNA probes having sequence homology to those mRNA species. Total nucleic acid was extracted from pools of unfertilized eggs, two-cell embryos, eight-cell embryos, and blastocysts. The nucleic acids were resolved electrophoretically, bound to diazotized paper following Northern transfer, and hybridized with 32P-labeled histone or actin DNA probes. Our findings demonstrate that there is a maternal store of histone and actin mRNA in the unfertilized egg but that this mRNA pool is reduced roughly 10-fold on an embryo basis by the mid-two-cell stage. Following this reduction of maternal mRNA, histone and actin mRNA accumulation from the eight-cell cleavage stage to the blastocyst increases proportionally to cell number and appears to be controlled by zygote genome transcription.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
May 1, 1979·Journal of Reproduction and Fertility·P R Braude, H R Pelham
Nov 1, 1979·Nature·P BraudeR Lobatto
Jun 1, 1978·Experimental Cell Research·S L Abreu, R L Brinster
May 1, 1978·Developmental Biology·I L LeveyR L Brinster
Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Oct 1, 1973·The Journal of Experimental Zoology·P J OldsJ D Biggers
Apr 1, 1982·Developmental Biology·A SodjaR S Zafar
Feb 1, 1980·Cell·E A FyrbergK L Kindle
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D B SittmanW F Marzluff
Aug 1, 1982·Developmental Biology·D S Durica, W R Crain
Jun 1, 1981·The Journal of Experimental Zoology·P L Kaye, R G Wales
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J Van Blerkom
Aug 1, 1981·Biological Reviews of the Cambridge Philosophical Society·M H Johnson
Jul 10, 1982·Nucleic Acids Research·A D Cooper, W R Crain
Jan 1, 1980·Developmental Biology·R Bachvarova, V De Leon
Jan 31, 1980·Nature·R L BrinsterM R Avarbock
Aug 1, 1981·Molecular and Cellular Biology·W R CrainK Van Doren

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Citations

Jul 1, 1997·Molecular Reproduction and Development·C L ChatotC A Ziomek
Aug 1, 1997·Molecular Reproduction and Development·S Bilodeau-Goeseels, G A Schultz
Oct 10, 1997·Molecular Reproduction and Development·J V FlemingW D Rees
May 1, 1990·Molecular Reproduction and Development·N A TelfordG A Schultz
Jul 1, 1990·Molecular Reproduction and Development·M B Dworkin, E Dworkin-Rastl
Apr 1, 1992·Molecular Reproduction and Development·A J WatsonG A Schultz
Dec 1, 1992·Molecular Reproduction and Development·A J Watson
Jan 1, 1993·Molecular Reproduction and Development·H MiyamotoM Nozaki
Feb 1, 1993·Molecular Reproduction and Development·A T SandsR J Schwartz
May 1, 1993·Molecular Reproduction and Development·S K PalG M Cooper
Feb 1, 1985·Developmental Biology·D H GiebelhausG A Schultz
Jun 1, 1988·Developmental Biology·S M LoboG Schatten
Feb 1, 1991·Developmental Biology·A A Poznanski, P G Calarco
Dec 1, 1992·Mutation Research·G A Schultz, S Heyner
May 1, 1993·Experimental Gerontology·J A MoshierA P Majumdar
Jul 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·W L Dean, G A Schultz
Mar 30, 1999·Biochimica Et Biophysica Acta·C E Watson, P L Davies
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P K ElderM J Getz
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A GravesG A Schultz
Nov 1, 1994·Zygote : the Biology of Gametes and Early Embryos·A AoA H Handyside
Mar 20, 2009·Cell Motility and the Cytoskeleton·Davina TondeleirAnja Lambrechts
Aug 30, 2005·Developmental Biology·Patrick AllardHugh J Clarke
Oct 1, 1986·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Sarah K Howlett
Jan 1, 1989·Molecular Reproduction and Development·P E NissonW R Crain
Jan 1, 1986·Teratogenesis, Carcinogenesis, and Mutagenesis·A C HahnelG A Schultz

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