Changes in the rate of carrier-mediated glucose transport by mouse mammary epithelial cells during ontogeny: hormone dependence delineated in vitro

Endocrinology
C G Prosser, Y J Topper

Abstract

Epithelial cells were isolated from mammary glands of mice in various physiological states, and the rate of carrier-mediated glucose transport was determined with 3-O-methylglucose. The basal rate (in the absence of exogenous insulin) increases about 40-fold as the animal of origin progresses from the virgin to the midlactating state and declines precipitously during involution. Insulin does not acutely stimulate carrier-mediated transport by cells isolated from virgin or lactating mice and evokes only about a 50% enhancement of this transport rate in the cells derived from pregnant and early postlactational animals. However, culture of mammary explants from pregnant mice in the presence of insulin, cortisol, and PRL before isolation of the epithelial cells results in a 400% increase in the basal rate of carrier-mediated glucose transport in the absence of exogenous hormones; this effect requires 3 days of incubation. The enhanced rate is equivalent to that in cells freshly isolated from animals in early lactation. Insulin-like growth factor I can mimic the chronic effect of insulin, but multiplication-stimulating activity, epidermal growth factor, and 20% fetal calf serum do not supplant insulin in this system. The maximum vel...Continue Reading

Citations

Jan 1, 1991·Comparative Biochemistry and Physiology. A, Comparative Physiology·S LavanderoM Sapag-Hagar
Aug 16, 1997·Biochimica Et Biophysica Acta·M C BarberR G Vernon
Jan 22, 2014·Journal of Animal Science and Biotechnology·Yong Shao, Feng-Qi Zhao
Nov 14, 2013·Journal of Mammary Gland Biology and Neoplasia·Feng-Qi Zhao
Dec 3, 2003·American Journal of Physiology. Cell Physiology·Changting XiaoJohn P Cant
Sep 1, 1987·Journal of Dairy Science·M F McGrath
Jun 13, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yong ShaoFeng-Qi Zhao
Jul 18, 2003·American Journal of Physiology. Cell Physiology·Changting Xiao, John P Cant
Jul 14, 2000·Physiological Reviews·D B Shennan, M Peaker

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