Changes in the refractive index of the stroma and its extrafibrillar matrix when the cornea swells

Biophysical Journal
Keith M MeekS Khan

Abstract

The transparency of the corneal stroma is critically dependent on the hydration of the tissue; if the cornea swells, light scattering increases. Although this scattering has been ascribed to the disruption caused to the arrangement of the collagen fibrils, theory predicts that light scattering could increase if there is an increased mismatch in the refractive indices of the collagen fibrils and the material between them. The purpose of this article is to use Gladstone and Dale's law of mixtures to calculate volume fractions for a number of different constituents in the stroma, and use these to show how the refractive indices of the stroma and its constituent extrafibrillar material would be expected to change as more solvent enters the tissue. Our calculations predict that solvent entering the extrafibrillar space causes a reduction in its refractive index, and hence a reduction in the overall refractive index of the bovine stroma according to the equation n'(s) = 1.335 + 0.04/(0.22 + 0.24 H'), where n'(s) is the refractive index and H' is the hydration of the swollen stroma. This expression is in reasonable agreement with our experimental measurements of refractive index versus hydration in bovine corneas. When the hydration o...Continue Reading

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Citations

Jul 3, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Cestmír CejkaJitka Cejková
Mar 13, 2008·Tissue Engineering. Part a·Rachael A B Crabb, Allison Hubel
Sep 25, 2008·Cornea·Sudi PatelJuan Rodriguez-Prats
Jun 20, 2007·Photochemistry and Photobiology·Cestmír CejkaJitka Cejková
Jul 22, 2008·Investigative Ophthalmology & Visual Science·Li DingKrystel R Huxlin
Jul 5, 2013·PloS One·Jingjing YouGerard Sutton
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Jan 16, 2013·Journal of Cataract and Refractive Surgery·Sudi PatelAlberto Artola
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