Changes in the regulatory form of Rhodospirillum rubrum nitrogenase as influenced by nutritional and environmental factors.

Journal of Bacteriology
D C Yoch, M Cantu

Abstract

The photosynthetic bacterium Rhodospirillum rubrum regulates the activity of its nitrogenase (N2ase) by interconverting the enzyme into three distinct enzymatic species: N2ase A (a fully active form) and two regulatory forms, N2ase Ractive and N2ase Rinactive. N2ase R is distinguished from N2ase A in vitro by the requirement of its Fe protein for activation by a Mn2+-dependent activating factor. N2ase is converted from the A to the R form in response to certain environmental factors such as carbon starvation, depletion of intracellular adenosine triphosphate, or the addition of NH4+ (or glutamate) to a culture of N-starved cells. The rapid inhibition of R. rubrum N2ase in vivo by NH4+ was shown to result from the conversion of N2ase A to N2ase Rinactive. On depletion of NH4+ from the culture, whole-cell N2ase activity returned; however, the enzyme remained in the R form. Unlike the effect of NH4+, adding glutamate to cells containing N2ase A did not inhibit in vivo activity, but converted the enzyme to the R form (N2ase Ractive). Although glutamate-induced N2ase R formation was much slower than the NH4+-induced reaction, it occurred in the presence of rifampin, indicating that de novo protein synthesis was not involved. This su...Continue Reading

References

Feb 1, 1977·Journal of Bacteriology·R A BenderB Magasanik
Dec 1, 1977·European Journal of Biochemistry·B C Johansson, H Gest
Feb 1, 1979·Journal of Bacteriology·R P CarithersD I Arnon
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P W Ludden, R H Burris
Oct 12, 1977·Biochimica Et Biophysica Acta·S NordlundH Baltscheffsky
Apr 27, 1978·Archives of Microbiology·W G Zumft, F Castillo
Dec 1, 1971·Journal of Bacteriology·A G ChapmanD E Atkinson
Mar 1, 1970·Journal of General Microbiology·J Drozd, J R Postgate
May 1, 1967·Journal of Bacteriology·J Gibson, S Morita

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Citations

Jan 1, 1992·Archives of Microbiology·A Soliman, S Nordlund
Oct 1, 1982·European Journal of Biochemistry·H HaakerC Veeger
Feb 1, 1985·Archives of Microbiology·J W Gotto, D C Yoch
Jan 1, 1987·Critical Reviews in Microbiology·P C Hallenbeck
Dec 1, 1981·Journal of Bacteriology·J K GordonW J Brill
Feb 1, 1981·Journal of Bacteriology·W J Sweet, R H Burris
Jan 9, 1992·Biochimica Et Biophysica Acta·P C Hallenbeck
Jan 1, 1985·Journal of Bacteriology·S NordlundP W Ludden
Jul 1, 1983·Annales de microbiologie·J C WillisonP M Vignais

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