Changes in the rumen bacterial community in response to sunflower oil and fish oil supplements in the diet of dairy sheep

Journal of Dairy Science
A BelenguerG Hervás

Abstract

Rumen microbial biohydrogenation of dietary unsaturated fatty acids has a major effect on the process of developing healthier dairy products. This study aimed to investigate in vivo the effect of diet supplementation with sunflower (SO) and fish (FO) oils on the rumen bacterial community in dairy sheep. First, 32 lactating ewes, divided in 8 lots of 4 animals each (2 lots per treatment), were fed a high-concentrate total mixed ration supplemented with 0, 2% SO, 1% FO, or 2% SO plus 1% FO. After 21 d, rumen fluid samples were taken from each lot for DNA extraction and fluorescence in situ hybridization (FISH) analysis. In a second experiment, 5 cannulated ewes were first fed the same TMR, with the exception of a higher forage level, and then changed to the same diet supplemented with 2% SO plus 1% FO. After 0, 3, and 10 d, rumen content samples were taken for DNA extraction and FISH analysis (fluid). Total bacteria and the Butyrivibrio group were studied in microbial DNA by terminal RFLP analysis (T-RFLP), and real-time PCR was used to quantify Butyrivibrio bacteria that produce vaccenic acid or stearic acid. In rumen fluid samples, total bacteria and clostridial clusters IX and XIV were analyzed by FISH. Dietary supplementation...Continue Reading

References

Jan 1, 1992·Applied and Environmental Microbiology·N O van GlyswykK Kulander
Jun 1, 1987·Applied and Environmental Microbiology·J L ViciniM P Bryant
Dec 1, 1972·Journal of General Microbiology·C G Orpin
Apr 1, 1993·International Journal of Systematic Bacteriology·L R KrumholzD A Stahl
Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsJ A Farrow
Aug 16, 1997·Biochimica Et Biophysica Acta·M C BarberR G Vernon
May 22, 2004·BioTechniques·Zhongtang Yu, Mark Morrison
Nov 3, 2005·Advances in Food and Nutrition Research·Donald L PalmquistDale E Bauman
Jun 14, 2006·The British Journal of Nutrition·I WasowskaR J Wallace
Oct 31, 2006·Antonie van Leeuwenhoek·Margarida R G MaiaR John Wallace
Dec 7, 2006·FEMS Microbiology Letters·R John WallaceDelphine Paillard
May 29, 2007·Veterinary Microbiology·Marisol CastilloJosep Gasa
Sep 4, 2007·Applied and Environmental Microbiology·Alvaro BelenguerHarry J Flint
Sep 5, 2008·International Journal of Systematic and Evolutionary Microbiology·Christina D MoonGraeme T Attwood
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
Feb 1, 2003·FEMS Microbiology Ecology·Tom C J HillBruce F Moffett
Dec 1, 2008·Animal : an International Journal of Animal Bioscience·M R F LeeN D Scollan
Jul 1, 2009·Animal : an International Journal of Animal Bioscience·C BoeckaertV Fievez

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Citations

Sep 13, 2011·Environmental Microbiology·Robert W LiMichael E Sparks
Apr 16, 2013·Journal of Animal Physiology and Animal Nutrition·A A AbuGhazaleh, A Ishlak
Apr 16, 2016·Journal of Animal Physiology and Animal Nutrition·L M M FerreiraK Osoro
Jan 8, 2018·Animal : an International Journal of Animal Bioscience·P CremonesiB Castiglioni
Jun 4, 2018·Journal of Dairy Science·Dipti W PittaGraeme Attwood
Jul 27, 2021·PloS One·Guillermo Martinez BoggioChristel Marie-Etancelin

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