PMID: 7013797Mar 17, 1981Paper

Changes in the solution structure of yeast phenylalanine transfer ribonucleic acid associated with aminoacylation and magnesium binding

Biochemistry
R O PottsM J Fournier

Abstract

The effect of aminoacylation on the structure of yeast phenylalanine tRNA was evaluated by laser light scattering. In these experiments, the translational diffusion coefficient (D20,w) of phenylalanyl-tRNA was monitored continuously during spontaneous deacylation in a variety of solution conditions. The results reveal that significant changes can occur in the hydrodynamic volume and electric charge as a consequence of aminoacylation but that the effects are magnesium dependent. At neutral pH, 20 degrees C, and 0.1 M salt, the D20,w value increased by 18% when deacylation occurred in 2--10 mM Mg2+ concentrations while no change in diffusivity was observed for tRNA deacylating in 0.5--1.0 mM Mg2+. The Mg2+ concentration dependence of the D20,w changes behaves in highly cooperative manner. The electric charges of aminoacyl-tRNA and nonacylated tRNA in 1 and 10 mM Mg2+ were estimated from the diffusive virial coefficients. In the higher Mg2+ conditions, aminoacyl-tRNA has a charge of 15 +/- 2e- while that of the nonacylated form is 10 +/- 2e-; both acylated and nonacylated tRNA have a charge of 11 +/- 4e- in 1 mM Mg2+. Taken together, the results indicate that aminoacylation permits the binding of additional Mg2+, resulting, in tur...Continue Reading

References

Jan 1, 1976·Annual Review of Biochemistry·A Rich, U L RajBhandary
Jul 5, 1977·Biochimica Et Biophysica Acta·Y Y Chao, D R Kearns
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M M Gottesman
Aug 23, 1976·Biochemical and Biophysical Research Communications·O PongsV A Erdmann
Nov 12, 1970·Biochimica Et Biophysica Acta·S K Chatterjee, H Kaji
Jan 14, 1969·Journal of Molecular Biology·M CohnM Grunberg-Manago
Jan 14, 1972·Journal of Molecular Biology·J J EnglanderS W Englander
Sep 18, 1973·Biochemical and Biophysical Research Communications·G J ThomasS C Mohr
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·Y P WongD R Kearns
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C R Jones, D R Kearns
Jun 1, 1967·Journal of Biochemistry·H Hashizume, K Imahori
Jan 1, 1971·Biochimie·G MelcherW Guschlbauer
Jun 4, 1971·Biochemical and Biophysical Research Communications·E Wickstrom
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R C Chin, C Kidson
Oct 15, 1971·Biochemical and Biophysical Research Communications·K Watanabe, K Imahori
Aug 16, 1965·Biochemical and Biophysical Research Communications·P S Sarin, P C Zamecnik
Feb 1, 1971·European Journal of Biochemistry·U J Hänggi, H G Zachau
Sep 7, 1970·FEBS Letters·A Danchin, M Grunberg-Manago

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Citations

Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J A Enríquez, G Attardi
Aug 2, 2005·Bioorganic & Medicinal Chemistry Letters·Sara N RichterManlio Palumbo
Sep 15, 2016·Nucleic Acids Research·Anne PlochowietzAchillefs N Kapanidis
Mar 13, 1990·Biochemistry·L S BehlenO C Uhlenbeck
Nov 27, 1990·Biochemistry·D E RuffnerO C Uhlenbeck
Sep 1, 2019·The Journal of Chemical Physics·Huan YangPaul C Whitford
May 2, 2020·The Journal of Physical Chemistry Letters·Qiwei YuOleg A Igoshin

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