Changes in the transcriptome of circulating immune cells of a New Zealand cohort with myalgic encephalomyelitis/chronic fatigue syndrome

International Journal of Immunopathology and Pharmacology
Eiren SweetmanWarren Tate

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a poorly understood disease affecting 0.2%-2% of the global population. To gain insight into the pathophysiology of ME/CFS in New Zealand, we examined the transcriptomes of peripheral blood mononuclear cells by RNA-seq analysis in a small well-characterized patient group (10 patients), with age/gender-matched healthy controls (10 control subjects). Twenty-seven gene transcripts were increased 1.5- to sixfold and six decreased three- to sixfold in the patient group ( P < 0.01). The top enhanced gene transcripts, IL8, NFΚBIA and TNFAIP3, are functionally related to inflammation, and significant changes were validated for IL8 and NFΚBIA by quantitative polymerase chain reaction (qPCR). Functional network analysis of the altered gene transcripts ( P < 0.01) detected interactions between the products related to inflammation, circadian clock function, metabolic dysregulation, cellular stress responses and mitochondrial function. Ingenuity pathway analysis ( P < 0.05) provided further insights into the dysfunctional physiology, highlighting stress and inflammation pathways. This analysis provides novel insights into the molecular changes in ME/CFS and contributes to the un...Continue Reading

References

Sep 20, 2008·Autoimmunity Reviews·Lorenzo LorussoGiovanni Ricevuti
Jun 27, 2009·BMC Medical Genomics·John W GowAbhijit Chaudhuri
Dec 4, 2009·Journal of Clinical Pathology·Lihan ZhangJonathan R Kerr
Aug 31, 2016·Proceedings of the National Academy of Sciences of the United States of America·Robert K NaviauxEric Gordon
Apr 19, 2018·Biochemical Society Transactions·Cara Tomas, Julia Newton

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Citations

Feb 3, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Peter L ToogoodDavid Hoffman
Aug 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Bindu D PaulSolomon H Snyder

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Methods Mentioned

BETA
RNA-seq
PCR

Software Mentioned

EdgeR
R
IPA
STRING
Bowtie
TopHat

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