Changes in tumor cell heterogeneity after chemotherapy treatment in a xenograft model of glioblastoma

Neuroscience
Alessandra M WelkerChristine E Beattie

Abstract

Glioblastoma (GBM) is a highly aggressive brain cancer with limited treatments and poor patient survival. GBM tumors are heterogeneous containing a complex mixture of dividing cells, differentiated cells, and cancer stem cells. It is unclear, however, how these different cell populations contribute to tumor growth or whether they exhibit differential responses to chemotherapy. Here we set out to address these questions using a zebrafish xenograft transplant model (Welker et al., 2016). We found that a small population of differentiated vimentin-positive tumor cells, but a majority of Sox2-positive putative cancer stem cells, were dividing during tumor growth. We also observed co-expression of Sox2 and GFAP, another suggested marker of glioma cancer stem cells, indicating that the putative cancer stem cells in GBM9 tumors expressed both of these markers. To determine how these different tumor cell populations responded to chemotherapy, we treated animals with temozolomide (TMZ) and assessed these cell populations immediately after treatment and 5 and 10days after treatment cessation. As expected we found a significant decrease in dividing cells after treatment. We also found a significant decrease in vimentin-positive cells, but...Continue Reading

Citations

Jan 23, 2019·Glia·Emma J van BodegravenElly M Hol
Oct 2, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Deanna M TiekRebecca B Riggins
Jun 10, 2020·Biomedicines·Verena J HerbenerHannah Strobel
Jun 1, 2018·Cancer Cell International·Hanin AlamirDeema Hussein
Aug 23, 2020·Nature Protocols·Chuan YanDavid M Langenau
May 12, 2020·Frontiers in Oncology·Giuseppe Nicolò FanelliCristian Scatena
Aug 31, 2019·Bulletin du cancer·Ludivine RabyPierre-Olivier Angrand

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