PMID: 8580517Jan 1, 1995Paper

Changes induced by zinc on thyroxine deiodination by rat liver in vivo and in vitro

Acta Physiologica, Pharmacologica Et Therapeutica Latinoamericana : órgano De La Asociación Latinoamericana De Ciencias Fisiológicas Y [de] La Asociación Latinoamericana De Farmacología
M Gonzalez PondalA A Zaninovich

Abstract

The effects of in vivo administration or in vitro addition of zinc on 5'-deiodination of thyroxine (T4) and the concentration of nonprotein sulfhydryl groups (NPSH) in rat liver were studied in 200-240 g body weight male Wistar rats. Twelve rats were injected i.p. with zinc sulphate 2 mg/kg body weight 24 h before the experiments were started. Animals were killed by cervical dislocation and the liver was immediately removed and homogenized. Dithiothreitol (DTT) (0, 2.5, 5 or 10 mM, final concentration) and 1 microCi 125I-T4 were added to homogenates. For the in vitro studies, animals were killed by cervical dislocation and the liver removed and added zinc or cadmium (2.5 or 5 mM) plus DTT and labelled T4. Homogenates were incubated for 90 min at 37 degrees C and thereafter chromatographed in Whatman 1 paper. Zinc-injected rats had a significant (P < 0.01) decrease in T4 deiodination and in the generation of iodine (P < 0.02) and T3 (P < 0.05). In the in vitro studies, both zinc and cadmium reduced (P < 0.02) the deiodination of T4, and the generation of iodine (P < 0.02) for zinc and P < 0.05 for cadmium) as well as the generation of T3 (p < 0.05). The NPSH in zinc-injected rats were within normal levels. Serum T4 and T3 in zin...Continue Reading

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