PMID: 26832697Feb 3, 2016Paper

Changes of CD4(+)Foxp3+regulatory T cells and CD4(+)IL-17+T cells in acrolein exposure rats

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
Ming WeiLu Yang

Abstract

To evaluate the changes of CD4(+) IL-17+T (Th17) and CD4(+)Foxp3+regulatory T (Treg) cells in peripheral blood and bronchoalveolar lavage fluid (BALF) , and therefore to explore the role of Th17 and Treg in acrolein exposure airway inflammation in rats. Forty male Wistar rats were randomly divided into 4 groups: a 2 wk acrolein exposure group, a 4 wk acrolein exposure group, a 2 wk control group and a 4 wk control group (n=10 each). Cells in BALF were collected and analyzed by absolute and differential cell counts.IL-17 and IL-6 levels in serum and BALF were tested by enzyme linked immunosorbent assay (ELISA). The proportion of CD4(+)IL-17+T and CD4(+) Foxp3+Treg in peripheral blood and BALF were determined by flow cytometry.The mRNA expressions of IL-17 and Foxp3 were measured by real-time PCR. Comparisons of the data between different groups were performed using one-way ANOVA, and SNK and Games-Howell test were used for comparison between 2 groups. Levels of IL-17 were remarkable increased in the 2 wk acrolein exposure group and the 4 wk acrolein exposure group in serum [ (52.64±1.89) ng/L, (76.73±5.57) ng/L], and BALF [ (79.07±5.67) ng/L, (96.61±6.44) ng/L] compared with the 2 wk control group [ (40.05±3.12) ng/L, (56.75±4.3...Continue Reading

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