Changes of cytokines and IgG antibody in chickens vaccinated with DNA vaccines encoding Eimeria acervulina lactate dehydrogenase

Veterinary Parasitology
Hongyan SongXiangrui Li

Abstract

The aim of this study was to investigate the changes of cytokines and specific serum IgG in chickens following vaccination with DNA vaccines encoding either Eimeria acervulina (E. acervulina) lactate dehydrogenase (LDH) antigen or LDH and chicken IL-2 or IFN-γ. Two-week-old chickens were randomly divided into five groups. Experimental group of chickens were immunized with DNA vaccines while control group of chickens were injected with pVAX1 plasmid alone or sterile water. All immunizations were boosted 2 weeks later. The LDH-specific IgG antibody response was measured at weeks 1-6 post-second immunization. The result showed that the antibody titers in chickens vaccinated with DNA vaccines were significantly different from those of the control groups 1 week after the second immunization (P<0.05) and reached the maximum values 3 weeks post-second immunization. The systemic and local cytokine mRNA expression was determined by quantitative RT-PCR 7 days post-second immunization. The specific IgG antibody levels against LDH of all chickens vaccinated with vaccines were increased compared to those of sterile water (H(2)O) and plasmid (pVAX1) control chickens 1-6 weeks post-second immunization (P<0.05). The mRNA levels of IFN-γ, IL-2,...Continue Reading

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Citations

Jan 9, 2016·Experimental Parasitology·Tran Duc HoanXiangrui Li
Sep 22, 2012·Molecular and Biochemical Parasitology·Huili ZhuXiangrui Li
Jan 23, 2016·Veterinary Parasitology·ZhenChao ZhangXiangRui Li
Dec 2, 2015·Vaccine·Marine MeunierDaniel Dory
Jul 17, 2015·Avian Pathology : Journal of the W.V.P.A·Jingwei HuangXiangrui Li
Dec 25, 2012·Research in Veterinary Science·Hongyan SongXiangrui Li
Oct 28, 2016·The Journal of Eukaryotic Microbiology·ZhenChao ZhangXiangRui Li
Jan 15, 2019·Parasitology Research·Shafiya Imtiaz RafiqiP S Banerjee
May 5, 2021·Parasite : Journal De La Société Française De Parasitologie·Huanzhi ZhaoHongyu Han

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