Changes of extracellular calcium concentration induced by application of excitatory amino acids in the human neocortex in vitro

Brain Research
A LückeE J Speckmann

Abstract

The influence of the glutamate subreceptor agonists N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) on cortical field potentials and on changes in extracellular free calcium concentration ([Ca2+]o) was tested on human neocortical slices (eleven from nine different patients). The tissue used was a small portion of that which is normally removed for the treatment of a brain tumor. [Ca2+]o and field potentials were measured by Ca(2+)-selective microelectrodes. Local pressure-microejection of NMDA (100 mumol/l)- and AMPA (1 mmol/l)-induced negative field potentials with maximal amplitudes of 0.9 +/- 0.1 mV (11 slices, mean +/- S.E.M.) and 1.0 +/- 0.1 mV (nine slices), respectively. The negative field potentials induced by NMDA were accompanied by monophasic decreases of [Ca2+]o (0.8 +/- 0.1 mmol/l, nine slices). AMPA elicited no (three slices) or only minor decreases of [Ca2+]o (0.2 +/- 0.1 mmol/l, five slices). The responses to the glutamate subreceptor agonists NMDA and AMPA were reversibly depressed by adding their specific antagonists DL-2-amino-5-phosphonovalerate (APV, 100 mumol/l, six slices) and 6-cyano-7-nitroquinoxalin-2,3-dion (CNQX, 5 mumol/l, four slices), respectively. T...Continue Reading

Citations

Jun 1, 1999·The International Journal of Eating Disorders·Y OzawaT Akama
Jun 7, 2006·Journal of Neuroscience Methods·Rüdiger Köhling, Massimo Avoli
Jun 30, 2001·Brain Research·A GorjiE J Speckmann
Aug 13, 1998·Glia·A Charles
Nov 13, 2004·Der Pathologe·D Sandmann-Keil, H Braak
Mar 11, 2000·Journal of Neurophysiology·M C WiestP R Montague
Jan 12, 2001·Physiological Reviews·E M Brown, R J MacLeod

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