Changes of glial-neuronal interaction and metabolism after a subconvulsive dose of pentylenetetrazole

Neurochemistry International
Haytham EloqayliUrsula Sonnewald

Abstract

Pentylenetetrazole (PTZ) injection causes seizures in rodents and this is used in several models of epilepsy. In the present study a low dose (20 mg/kg) was injected into rats in order to analyze metabolic disturbances caused by subconvulsive amounts of PTZ. Intraperitoneal injection of PTZ was followed, 30 min later, by injection of [1-(13C)]glucose plus [1,2-(13C)]acetate and 15 min thereafter decapitation. Analyses of extracts from cerebrum, subcortex and cerebellum were performed using 13C NMRS and HPLC. Whereas convulsive doses of PTZ lead to most pronounced changes in cerebellum [J. Neurochem. 85 (2003) 1200], it could be shown that subconvulsive doses affected mainly amino acid metabolism in cerebrum. In glutamatergic neurons in the cerebrum PTZ affected both the metabolic and releasable pools of glutamate, whereas, in the subcortex and cerebellum only the metabolic pool was affected. This could be deducted from the findings that less [4-(13C)]glutamine, [3,4-(13C)]glutamate and [2-(13C)]aspartate, which are labeled from [1-(13C)]glucose, were detected in this area. Glial metabolism was also changed as evidenced by the decreased pyruvate carboxylation versus pyruvate dehydrogenation ratio both in cerebrum and subcortex. ...Continue Reading

References

Feb 2, 1979·Brain Research·M D Norenberg, A Martinez-Hernandez
Jun 1, 1970·The Journal of Nervous and Mental Disease·E A Rodin, H D Calhoun
Dec 1, 1983·Neuropharmacology·H Lal, M W Emmett-Oglesby
Jan 1, 1996·Developmental Neuroscience·A TaylorH Bachelard
Dec 9, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·B HasselU Sonnewald
Mar 14, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·U SonnewaldA Schousboe
Nov 11, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A HåbergU Sonnewald
Dec 9, 2000·Developmental Neuroscience·H QuU Sonnewald
Feb 1, 2002·Neurochemistry International·Daniel KondziellaUrsula Sonnewald
Sep 3, 2002·Neuroscience and Biobehavioral Reviews·Marianna E JungMichael B Gatch
Feb 11, 2003·Epilepsy Research·Marc YudkoffItzhak Nissim
May 20, 2003·Journal of Neurochemistry·Haytham EloqayliUrsula Sonnewald
Aug 2, 2003·Neurochemistry International·Daniel KondziellaUrsula Sonnewald
Oct 29, 2003·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Helle S WaagepetersenArne Schousboe

❮ Previous
Next ❯

Citations

Sep 16, 2010·Neurochemical Research·Ursula Sonnewald, Caroline Rae
Oct 25, 2011·Neuron Glia Biology·Jan AlbrechtMichael Aschner
Nov 17, 2009·Neurochemistry International·Stacey Carmody, Lorraine Brennan
Sep 19, 2014·Journal of Neurochemistry·Isaac Marin-ValenciaCraig R Malloy
Mar 31, 2007·Seizure : the Journal of the British Epilepsy Association·R Uribe-EscamillaA Alfaro-Rodríguez
Nov 26, 2010·Epilepsy Research·Selene LomoioElda Scherini
Jul 4, 2018·Endocrine-related Cancer·James F PowersArthur S Tischler
Feb 9, 2019·Journal of Inherited Metabolic Disease·Alfonso Oyarzabal, Isaac Marin-Valencia
Oct 12, 2018·Endocrine-related Cancer·James F PowersArthur S Tischler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.