PMID: 18724007Aug 30, 2008Paper

Changes of HCN gene expression and I(f) currents in Nkx2.5-positive cardiomyocytes derived from murine embryonic stem cells during differentiation

Biomedical Research
Shuichi YanoIchiro Hisatome

Abstract

Changes in the expression of hyperpolarization-activated cyclic nucleotide (HCN)-gated channels and I(f) currents during the differentiation of embryonic stem cells into cardiac cells remain unknown. We examined changes of HCN genes in expression and function during the differentiation of Nkx2.5-positive cardiac precursor cells derived from mouse ES cells using cell sorting, RTPCR, immunofluorescence and whole cell patch-clamp techniques. Cs(+)-induced inhibition of automaticity and transcription of HCN genes increased during differentiation. Expressions of Nkx2.5, a marker of cardiac progenitor cell, and Flk1, a marker of hemangioblast, were mutually exclusive. Messenger RNA and proteins encoded by HCN1 and 4 genes were predominantly observed in Nkx2.5-positive cells on day 15, although Flk1-positive cells did not express genes of the HCN family on that day. Cs(+)-induced prolongation of the cycle of spontaneous action potentials and I(f) currents were predominantly observed on day 15. These results suggested that a fraction of Nkx2.5-positive cardiac precursor cells was committed to pacemaking cells expressing I(f) channels predominantly encoded by HCN 1 and 4 genes.

References

Jan 1, 1985·Progress in Biophysics and Molecular Biology·D DiFrancesco
Jan 1, 1993·Physiological Reviews·H IrisawaW Giles
Feb 1, 1997·Pflügers Archiv : European journal of physiology·R B RobinsonI S Cohen
Oct 19, 1999·Biochimica Et Biophysica Acta·T VaccariD DiFrancesco
May 31, 2001·The Journal of General Physiology·C AltomareD DiFrancesco
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kyoko HidakaTakayuki Morisaki
Mar 11, 2005·Molecular Biology of the Cell·Annabelle MéryMichel Pucéat
Jul 22, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun K YamashitaShin-Ichi Nishikawa
Oct 1, 2005·Circulation Journal : Official Journal of the Japanese Circulation Society·Einosuke MizutaIchiro Hisatome
Oct 24, 2006·Biochemical and Biophysical Research Communications·Einosuke MizutaIchiro Hisatome
Jan 20, 2007·Circulation Research·Mathilda T M MommersteegVincent M Christoffels

❮ Previous
Next ❯

Citations

Feb 5, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ying WangAlvaro Puga
Feb 5, 2010·American Journal of Physiology. Cell Physiology·Andrew P HegleEric A Accili
Mar 5, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Midori IidaTatsutoshi Nakahata
Sep 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Natig GassanovUta C Hoppe
Apr 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irit HuberLior Gepstein
Jul 22, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun K YamashitaShin-Ichi Nishikawa
Dec 2, 2020·International Journal of Molecular Sciences·Junjun LiYoshiki Sawa
Jan 10, 2020·Advanced Drug Delivery Reviews·Yimu ZhaoMilica Radisic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.