Changes of nuclear protein kinase C activity and isotype composition in PC12 cell proliferation and differentiation

Experimental Cell Research
P BorgattiS Capitani

Abstract

To establish whether protein kinase C was involved in the nuclear events underlying cell differentiation and proliferation, rat pheochromocytoma PC12 cells, serum-starved for 24 h, were treated with either differentiating doses of nerve growth factor or high serum concentrations, which represented a powerful mitogenic stimulus. Western blot analysis with isoform-specific antibodies, performed on whole cell homogenates, cytoplasms, and purified nuclei, showed that PKC isotypes alpha, beta I, beta II, delta, epsilon, eta, and zeta were expressed in PC12 cells and that all of them, except for beta I, were found at the nuclear level, variably modulated depending on the cell treatment. Compared to serum-stimulated cells, in which an early (1 day) and marked rise of protein kinase C activity was followed by a plateau, nerve growth factor-treated cells showed a progressive increase of protein kinase C activity coincident with the onset and maintenance of the differentiated phenotype. Western blot analysis of nuclei isolated from fully differentiated cells demonstrated an increase of protein kinase C alpha, paralleled by enhanced phosphotransferase activity along with the nerve growth factor treatment, and complete loss of the delta is...Continue Reading

Citations

Oct 31, 2012·International Journal of Molecular Sciences·Daniela Cosentino-GomesJosé Roberto Meyer-Fernandes
Mar 3, 2009·Biochemical and Biophysical Research Communications·Renhao XuePeng Chen
Mar 8, 2006·Phytotherapy Research : PTR·Cheorl-Ho KimIn-Seon Lee
Aug 10, 2006·Journal of Cellular Physiology·Camilla EvangelistiAlberto M Martelli
Sep 1, 2004·Cellular Signalling·Giovanna TabelliniAlberto Maria Martelli
Sep 16, 1999·Biochemical and Biophysical Research Communications·D S DesaiS Ohno
Oct 23, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mingcai ZhaoMichael P Walsh
Mar 22, 2002·The Journal of Pharmacology and Experimental Therapeutics·Alok DeDipak K Sarkar
Mar 25, 2009·International Journal of Immunopathology and Pharmacology·P SecchieroG Zauli
Nov 14, 1997·The American Journal of Physiology·W H ZhuD E Millhorn
May 31, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rebecca VoltanGiorgio Zauli
Aug 17, 2000·Biochemical and Biophysical Research Communications·B SparatoreE Melloni
Dec 19, 2000·Biochemical and Biophysical Research Communications·B SparatoreE Melloni
Dec 1, 2001·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J D LampassoR Dziak
Jun 12, 2003·Experimental Cell Research·Giovanna TabelliniAlberto M Martelli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.