Changes of Ocular Surface and the Inflammatory Response in a Rabbit Model of Short-Term Exposure Keratopathy

PloS One
Chun-Ting LaiWei-Li Chen

Abstract

To evaluate the ocular surface change and the inflammatory response in a rabbit model of short-term exposure keratopathy. Short term exposure keratopathy by continuous eyelid opening was induced in New Zealand white rabbits for up to 4 hours. Ultrasound pachymetry was used to detect central total corneal thickness. In vivo confocal microscopy and impression cytology were performed to evaluate the morphology of ocular surface epithelium and the infiltration of inflammatory cells. Immunohistochemistry for macrophage,neutrophil, CD4(+) T cells, and CD8(+) T cells were performed to classify the inflammatory cells. Scanning electron microscopy(SEM) was performed to detect ocular surface change.The concentrations of IL-8, IL-17, Line and TNF-αwere analyzed by multiplex immunobead assay. TUNEL staining was performed to detect cellular apoptosis. Significant decrease ofcentral total cornealthickness were found within the first 5 minutes and remained stable thereafter, while there were no changes of corneal epithelial thickness.No significant change of corneal, limbal and conjunctival epithelial morphology was found by in vivo confocal microscopy except the time dependent increase of superficial cellular defects in the central cornea. I...Continue Reading

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Citations

Apr 12, 2019·Journal of Cellular and Molecular Medicine·Guoliang WangCheng Li
Nov 22, 2017·Oxidative Medicine and Cellular Longevity·Evgeni Yu ZerniiIvan I Senin
May 19, 2021·Translational Vision Science & Technology·Xiao-Min ChenShi-You Zhou

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Methods Mentioned

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scanning electron microscopy
confocal microscopy
fluorescence microscopy
confocal

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