Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging

PloS One
Augusto SchneiderMichal M Masternak

Abstract

The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 months of age for miRNA sequencing. We detected a total of 404 miRNAs in the ovarian samples, from which the abundance of 22 and 33 miRNAs changed with age in N and df/df mice, respectively. Of these, only three miRNAs were commonly regulated with age between N and df/df mice, indicating a very divergent miRNA profile between genotypes. We also detected that 46 miRNAs were regulated between N and df/df mice, of which 23 were regulated exclusively in young mice, 12 exclusively in old mice and 12 commonly regulated at young and old ages. Many genes likely to be targeted by these miRNAs are involved in the FoxO, mTOR, PI3k/Akt and insulin signaling pathways. These results suggest that the aging process has a differential impact on the ovarian miRNA profile in df/df mice, and suggest that these miRNAs can be central players in the maintenance of a younger ovarian phenotype.

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Citations

Feb 11, 2020·Klinicheskaia laboratornaia diagnostika·S V BulgakovaS G Gorelik
Jan 25, 2019·Aging·Jinjin ZhangShixuan Wang
Jan 20, 2020·Aging·Marcelo B CavalcanteMichal M Masternak
Nov 30, 2019·BioMed Research International·Xiaoyu XiYanzhi Jiang
Nov 28, 2019·Oxidative Medicine and Cellular Longevity·F Timóteo-FerreiraE Silva
May 15, 2020·Reproductive Biology and Endocrinology : RB&E·Xi XiaAmanda Kallen
Oct 25, 2020·Reproduction in Domestic Animals = Zuchthygiene·Pengyan SongChenyu Tao
Jul 16, 2020·Molecular and Cellular Endocrinology·Runju ZhangAmanda N Kallen
Apr 14, 2021·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Joseph M DhahbiMichal M Masternak
Aug 14, 2021·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Joseph M DhahbiMichal M Masternak

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Datasets Mentioned

BETA
GSE87518

Methods Mentioned

BETA
blood collection
Chip
PCR
miRNA-Seq
RNASeq

Software Mentioned

EdgeR
sRNAtoolbox
mirPath
R
RNASeq
DESeq
Bioconductor

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