PMID: 8605239Feb 9, 1996Paper

Changes of phage T7 nucleoprotein structure at low ionic strength. A Raman spectroscopic study

Biochimica Et Biophysica Acta
P GrófG Rontó

Abstract

To detect changes in DNA and/or protein structures of phage T7 under different ionic strength, Raman spectra of phage T7 have been recorded in solutions of three different NaCl + Tris concentrations. Iterative Jansson-Van Cittert deconvolution, as well as decomposition methods have been used to quantify changes in DNA structure. Significant modifications in ratios of contributions from 675 and 685 per cm vibrations, as well as in the DNA backbone vibrations, characteristic for B-DNA, near 835 per cm frequency have been found. Changes of the base electronic structure were identified in the interval between 1280 and 1400 cm(-1). Estimation of the overall protein structure suggests predominant beta-sheet content.

References

Mar 1, 1978·Nucleic Acids Research·D C Goodwin, J Brahms
Apr 8, 1992·Biochimica Et Biophysica Acta·J Bandekar
Nov 1, 1991·Biochimie·D AslanianJ Massoulié
Jan 1, 1988·European Biophysics Journal : EBJ·G RontóE V Shtikova
Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S C ErfurthW L Peticolas
Jun 5, 1983·Journal of Molecular Biology·R W Williams
May 18, 1995·Biochimica Et Biophysica Acta·D AslanianP Masson

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Citations

Mar 31, 1998·Journal of Pharmaceutical Sciences·C R MiddaughD R Casimiro

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