Changes of PKA and PDK1 in the principal piece of boar spermatozoa treated with a cell-permeable cAMP analog to induce flagellar hyperactivation

Molecular Reproduction and Development
H Harayama, Kazumi Nakamura

Abstract

A cAMP-induced protein tyrosine phosphorylation and flagellar hyperactivation are controlled via complicated signaling cascades in mammalian spermatozoa. For instance, these events seem to be regulated positively by the PKA-mediated signaling and negatively by the PI3K/PDK1-mediated signaling. In this article, we have shown molecular changes of PKA and PDK1 in cAMP analog (cBiMPS)-treated boar spermatozoa in order to disclose possible roles of these kinases in protein tyrosine phosphorylation and hyperactivation. Ejaculated spermatozoa were incubated with cBiMPS, and then they were used for biochemical analyses of sperm kinases by Western blotting and indirect immunofluorescence and for assessment of flagellar movement. The first 30-min incubation with cBiMPS highly activated PKA of the principal piece to the accompaniment of autophosphorylation on Thr-197 of catalytic subunits. However, protein tyrosine phosphorylation and hyperactivation were fully induced in the sperm samples after the 180-min incubation. A potentially active form of PDK1 (54/55-kDa phospho-PDK1) was detected in the principal piece of the spermatozoa during the 90-min incubation. Another potentially active form (59-kDa phospho-PDK1) gradually increased durin...Continue Reading

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Citations

Mar 20, 2012·Cell and Tissue Research·Janetti SignorelliPatricio Morales
Jan 28, 2010·The Journal of Reproduction and Development·Shunsuke TateHiroshi Harayama
Jul 19, 2015·General and Comparative Endocrinology·B R Lackey, S L Gray
Aug 22, 2015·Reproduction in Domestic Animals = Zuchthygiene·D K Gangwar, S K Atreja
Nov 25, 2016·Molecular Reproduction and Development·Mariko TsukamotoHiroshi Iida
Nov 1, 2018·Reproductive Medicine and Biology·Hiroshi Harayama
May 11, 2019·Molecular Reproduction and Development·Beatriz Macías-GarcíaLauro González-Fernández
Mar 7, 2020·Cell and Tissue Research·Michal ZigoPeter Sutovsky

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